Room temperature solvent extraction for simple and fast determination of total concentration of Ca, Cu, Fe, Mg, Mn, and Zn in bee pollen by FAAS along with assessment of the bioaccessible fraction of these elements using in vitro gastrointestinal digestion.

Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)
Pawel PohlPiotr Jamroz

Abstract

Bee pollen is recognized to be a source of different nutrients, including minerals. As a food supplement, its quality and safety due to concentrations of essential macro- and microelements, and harmful trace elements has to be verified. Fast and simple element analysis of bee-collected pollen can be regarded as an important part of its quality assurance and control. The present study aimed at developping a new method for determination of selected elements (Ca, Cu, Fe, Mg, Mn, Zn) of bee pollen based on solvent extraction and completely avoiding a high temperature treatment with concentrated reagents. In addition, in vitro gastrointestinal digestion was used to assess bioavailability of elements from this food supplement. Bee pollen samples were dried and pulverized. Total concentrations of Ca, Cu, Fe, Mg, Mn, and Zn were determined by flame atomic absorption spectrometry (FAAS) in sample solutions obtained by wet digestion (WD) in concentrated HNO3 or alternatively by solvent extraction (SE) with diluted solutions of HNO3. Gastrointestinal digestion was mimicked using simulated solutions of gastric and intestinal juices followed by determination of Ca, Cu, Fe, Mg, Mn and Zn concentrations in the bioaccessible fraction by FAAS. ...Continue Reading

Citations

Jan 30, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Dongyuan WangHongqing Wang
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eliza MatuszewskaJan Matysiak
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Željka Peršurić, Sandra Kraljević Pavelić
Aug 21, 2021·Frontiers in Nutrition·Zhongke SunChengwei Li

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