Room-temperature vibrational difference spectrum for S2QB-/S1QB of photosystem II determined by time-resolved Fourier transform infrared spectroscopy

Biochemistry
H ZhangT Wydrzynski

Abstract

Time-resolved FTIR spectroscopy has been used to kinetically characterize the vibrational properties of intact photosystem II-enriched membrane samples undergoing the S1QB-to-S2QB- transition at room temperature. To optimize the experimental conditions for the FTIR measurements, oxygen polarographic and variable chlorophyll a fluorescence measurements were used to define the decay of S2 and QA-, respectively. The flash-induced S2QB-/S1QB difference spectra were measured at a temporal resolution of 4.44 s and a spectral resolution of 4 cm-1. An intense positive band is observed at 1480 cm-1 in the difference spectrum and shows a slow decay with a half time of approximately 13 s. Based on its decay kinetics and analogy to the infrared absorption of QA- of photosystem II and QB- in bacterial reaction centers, we conclude that the 1480 cm-1 band arises from QB- of PSII and tentatively assign it to the upsilon(CO) mode of the semiquinone anion QB-. The infrared spectral features attributed to the S1-to-S2 transition of the Mn cluster at room temperature show striking similarity to the S2/S1 difference spectrum measured at cryogenic temperatures (Noguchi, T., Ono, T.-A., and Inoue, Y. (1995) Biochim. Biophys. Acta 1228, 189-200).

Citations

Sep 5, 2008·Photosynthesis Research· Govindjee
Jan 20, 2004·European Journal of Biochemistry·André RemyKlaus Gerwert
Apr 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bridgette A BarryR Brian Dyer
Jul 21, 2014·Photosynthesis Research·Johannes MessingerG Charles Dismukes
Jun 30, 2005·Biophysical Journal·Bridgette A BarryDavid L Jenson
Jul 30, 2003·Biophysical Journal·Kelly M Halverson, Bridgette A Barry
Sep 26, 2003·Biophysical Journal·Kelly M Halverson, Bridgette A Barry
Dec 15, 2000·Biochimica Et Biophysica Acta·H A ChuG T Babcock
Apr 22, 2004·Biochimica Et Biophysica Acta·Idelisa Pujols-Ayala, Bridgette A Barry
Sep 30, 2016·Photosynthesis Research·Alberto Mezzetti, Winfried Leibl
Nov 28, 2018·Photosynthesis Research·Brendon ConlanJohannes Messinger

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