Root Exudate-Induced Promoter Activity in Pseudomonas fluorescens Mutants in the Wheat Rhizosphere.

Applied and Environmental Microbiology
L S van Overbeek, J D van Elsas

Abstract

Tn5-B20 (lacZ as reporter gene) transcriptional fusion mutants of Pseudomonas fluorescens R2f were screened for their response to wheat root exudate. Several mutants showed (beta)-galactosidase activity under the influence of wheat root exudate. In one such mutant, RIWE8, gene expression was specifically induced by proline but not by 125 other substrates. This mutant also showed reporter gene induction, albeit to a lesser extent, by exudate of maize and grass roots but not by that of clover roots. In situ promoter activity of RIWE8 was found in Flevo silt loam soil amended with proline but not in water-, arginine-, glutamic acid-, or malic acid-amended soils. Reporter gene expression in RIWE8 was triggered in a model rhizosphere-soil system in the presence of wheat, maize, and grass roots but not in the presence of clover roots or in root-free (bulk) soil. The induction of expression of the reporter gene in soil, using this approach, is suggestive that promoter activity in RIWE8 may be useful for the construction of organisms with rhizosphere-controlled beneficial genes.

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Citations

May 29, 1999·Applied and Environmental Microbiology·C H JaegerM K Firestone
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·R M BringhurstD J Gage
Feb 19, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·M SreevidyaR K Varshney
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Feb 24, 2001·Environmental Microbiology·R J EllisM J Bailey
Mar 1, 2003·Archives of Microbiology·Avinash SonawaneKlaus-Heinrich Röhm
Sep 10, 2005·Applied and Environmental Microbiology·Stéphane CompantEssaïd Ait Barka
Jun 23, 2011·FEMS Microbiology Ecology·Ulisses Nunes da RochaLeonard Simon van Overbeek
May 1, 1997·Applied and Environmental Microbiology·K M NielsenJ D van Elsas
Nov 15, 1997·Applied and Environmental Microbiology·A GaballaN Koedam
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