Root Proteomics Reveals the Effects of Wood Vinegar on Wheat Growth and Subsequent Tolerance to Drought Stress

International Journal of Molecular Sciences
Yuying WangYihong Ge

Abstract

Wood vinegar (WV) or pyroligneous acid (PA) is a reddish-brown liquid created during the dry distillation of biomass, a process called pyrolysis. WV contains important biologically active components, which can enhance plant growth and tolerance to drought stress. However, its mechanism of action remains unknown. Our results after presoaking wheat seeds with various concentrations of WV indicate that a 1:900 WV concentration can significantly enhance growth. To investigate the response of wheat roots to drought stress, we compared quantitative proteomic profiles in the roots of wheat plants grown from seeds either presoaked (treatment) or non-presoaked (control) with WV. Our results indicated that the abscisic acid (ABA) content of wheat roots in the WV treatment was significantly increased. Reactive oxygen species (ROS) and malonaldehyde (MDA) levels roots were significantly lower than in the control treatment under drought stress, while the activity of major antioxidant enzymes was significantly increased. Two-dimensional electrophoresis (2D-PAGE) identified 138 differentially accumulated protein (DAP) spots representing 103 unique protein species responding to drought stress in wheat roots of the control and WV-treated groups...Continue Reading

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Citations

Apr 4, 2019·International Journal of Molecular Sciences·Shuping WangGaisheng Zhang
Mar 15, 2020·Environmental Science and Pollution Research International·Bin WangJianyong Zhang
Aug 18, 2020·Ecotoxicology and Environmental Safety·Zuzana FačkovcováStefano Loppi

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Datasets Mentioned

BETA
KX711890.1
EMS54484.1
AK457377
EMS61931.1
AJ414697
CAC94001.1
AK457420
ABL74258.1

Methods Mentioned

BETA
PCR
MDA
electrophoresis
protein folding
environmental stress
Protein Assay

Software Mentioned

Originlab
PDQuest
TMpred
Beacon [UNK]
STRING
Mascot
BiNGO
Cytoscape
Oligo
SPSS

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