Abstract
Ropinirole (ROP) is a dopamine agonist that has been used as therapy for Parkinson's disease. In the present study, we aimed to detect whether gene expression was modulated by ROP in SH-SY5Y cells. SH-SY5Y cell lines were treated with 10 µM ROP for 2 h, after which total RNA was extracted for whole genome analysis. Gene expression profiling revealed that 113 genes were differentially expressed after ROP treatment compared with control cells. Further pathway analysis revealed modulation of the phosphatidylinositol 3-kinase (PI3K) signaling pathway, with prominent upregulation of PIK3C2B. Moreover, batches of regulated genes, including PIK3C2B, were found to be located on chromosome 1. These findings were validated by quantitative RT-PCR and Western blot analysis. Our study, therefore, revealed that ROP altered gene expression in SH-SY5Y cells, and future investigation of PIK3C2B and other loci on chromosome 1 may provide long-term implications for identifying novel target genes of Parkinson's disease.
References
Sep 28, 1998·Annals of Neurology·C W OlanowD Brooks
Aug 14, 1999·Brain Research·M IidaN Ogawa
Oct 10, 2002·FEBS Letters·Dora VisnjićHrvoje Banfić
Dec 5, 2002·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·A H V Schapira
Apr 1, 2003·Annals of Neurology·Anthony H V Schapira, C Warren Olanow
Apr 23, 2003·Trends in Pharmacological Sciences·Silvia MandelMoussa B H Youdim
Jun 5, 2004·Biochemical and Biophysical Research Communications·Jikun ZhaHong-Bing Shu
Mar 3, 2005·Neuroscience Letters·Tianhong PanWeidong Le
Apr 1, 2005·The American Journal of Psychiatry·Abhay SinghJames Jacobson
Jun 2, 2005·The Journal of Cell Biology·Tania MaffucciMarco Falasca
Nov 30, 2005·Molecular and Cellular Biology·Kazutoshi HaradaPaul A Khavari
Jul 22, 2006·Advances in Enzyme Regulation·Aleksandra SindićHrvoje Banfić
Jul 25, 2006·Nature Cell Biology·Lara FallonEdward A Fon
Aug 2, 2006·Nature Cell Biology·Koraljka Husnjak, Ivan Dikic
Aug 19, 2006·Human Mutation·Yonghong LiAndrew Grupe
Oct 5, 2007·Neuromolecular Medicine·D CaccamoP Calabresi
Jan 19, 2008·Neuroscience Letters·Kazuhiro NakasoKenji Nakashima
Jan 24, 2009·Apoptosis : an International Journal on Programmed Cell Death·Oren A LevyLloyd A Greene
Feb 28, 2009·Neuroimmunomodulation·Xiaolan FanWeidong Le
Apr 29, 2009·Clinical Science·Anne FougeratMuriel Laffargue
Jul 5, 2011·Neurología : publicación oficial de la Sociedad Española de Neurología·A Alonso CánovasJ C Martínez Castrillo