ROS and Oxidative Stress Are Elevated in Mitosis during Asynchronous Cell Cycle Progression and Are Exacerbated by Mitotic Arrest

Cell Systems
Jesse C PattersonMichael B Yaffe

Abstract

Although elevated levels of reactive oxygen species (ROS) have been observed in cancer cells and cancer cells aberrantly proliferate, it is not known whether the level of reactive oxygen species and the accumulation of oxidative damage to macromolecules vary across the cell cycle. Here, we measure the prevalence of reactive oxygen species and of biomolecule oxidation across the cell cycle in freely cycling cancer cells. We report that reactive oxygen species vary during the cell cycle and peak in mitosis, resulting in mitotic accumulation of oxidized protein cysteine residues. Prolonged mitotic arrest further increased the levels of ROS and the abundance of oxidatively damaged biomolecules, including cysteine-sulfenic-acid-containing proteins and 8-oxoguanine. These finding suggest that mitotic arrest agents may enhance the effects of ROS-dependent anticancer therapies.

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Citations

Jun 9, 2019·The Journal of Biological Chemistry·Joseph Y Ong, Jorge Z Torres
Jul 23, 2020·Science Signaling·Daniel C LimMichael B Yaffe
Sep 4, 2020·Cancer Research·Khadijeh Alnajjar, Joann B Sweasy
Jul 16, 2019·Cell Systems·Jesse C PattersonMichael B Yaffe
Dec 21, 2020·Redox Biology·Haya AlshaabiBrian Cunniff
Nov 8, 2020·Molecular Cancer Therapeutics·Joshua T KowalczykMarielle E Yohe
Nov 26, 2020·Molecular & Cellular Oncology·Daniel C LimMichael B Yaffe
Feb 11, 2021·International Journal of Molecular Sciences·Jakub RokDorota Wrześniok
Mar 11, 2021·Free Radical Biology & Medicine·José-Luis García-GiménezFederico V Pallardó
Apr 4, 2021·Biomolecules·Meytal RadzinskiDana Reichmann
Jul 3, 2021·International Journal of Molecular Sciences·Cuihua LiuBing Wang
Jul 3, 2021·International Journal of Molecular Sciences·Christine HanselDiana Klein
Jul 22, 2021·The Journal of Cell Biology·Nicolas TavernierLionel Pintard
Oct 2, 2021·Cancer Research·Kumar SanjivUlrika Warpman Berglund
Dec 21, 2021·Expert Opinion on Drug Discovery·Robert MusiolJacek Mularski

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