Abstract
In the current study, the effects of the reactive oxygen species (ROS) generator 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) on extracellular and intracellular ROS production in human keratinocytes (HACAT) were studied. AAPH is a water-soluble compound able to generate ROS at known and constant rates at 37°C. The short treatment (2 h) with AAPH brought a significant dose-dependent increase in NADPH oxidase activity in intact keratinocytes. The long-term treatment (24 h) with AAPH led to a persistent increase in NADPH oxidase activity for up to 48 hour following the AAPH removal from cell incubation medium. ROS and nitric oxide levels, lipoperoxidation, intracellular calcium, mitochondrial superoxide production, and membrane potential were significantly modified in AAPH-treated HACAT. Superoxide dismutase (SOD) and/or catalase addition to HACAT revealed that untreated keratinocytes produce mostly superoxide anion (O 2- ), while AAPH-treated keratinocytes overproduce hydrogen peroxide (H 2 O 2 ) in extracellular medium. H 2 O 2 is particularly stable and plays important roles in several cell signaling pathways. Taken together, our findings suggest a cost-effective and easily reproducible in vitro model of stressed human ...Continue Reading
References
Mar 5, 2004·Chemistry and Physics of Lipids·Yong CuiKyoung-Chan Park
Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser
Mar 14, 2009·Journal of Dermatological Science·Francesca PrignanoTorello Lotti
Mar 8, 2011·Pigment Cell & Melanoma Research·Tomohisa Hirobe
May 12, 2011·Journal of Pharmaceutical Sciences·Jay WerberJunyan A Ji
May 11, 2012·Dermatology : International Journal for Clinical and Investigative Dermatology·Luyan TangWenwen Fu
May 29, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K J Senthil KumarYou-Cheng Hseu
Jan 15, 2014·Journal of Cellular and Molecular Medicine·Matteo BecattiNiccolò Taddei
Jan 28, 2015·Molecular Medicine Reports·Cuiping GuanAie Xu
Jun 9, 2015·Journal of Dermatological Science·Victoria BaryginaClaudia Fiorillo
Aug 22, 2015·Redox Biology·Agnes GörlachOlga Krizanova
Mar 8, 2016·International Journal of Molecular Sciences·Hafiz Maher Ali ZeeshanHan-Jung Chae
Oct 11, 2016·Archives of Dermatological Research·Jian LiQin-Ping Yang
Jan 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan WangZhen Yang
Citations
Jun 24, 2019·Photochemistry and Photobiology·Zhenbang XiaoZhong-Ji Qian
Jun 2, 2020·Frontiers in Medicine·Giovanna G AltobelliVincenzo Cimini
Aug 18, 2020·Drug Delivery and Translational Research·Patrícia Mazureki CamposMaria Vitória Lopes Badra Bentley
Aug 11, 2020·Free Radical Biology & Medicine·Camilo López-AlarcónMichael J Davies
Jan 28, 2021·International Journal of Nanomedicine·Xiaolin HuQuanshun Li
Aug 6, 2021·Food & Function·Wei SongHai-Bo Yang
Aug 2, 2021·Pigment Cell & Melanoma Research·Bo Xie, Xiuzu Song
Aug 14, 2021·European Journal of Medicinal Chemistry·Zhonglei HeJames F Curtin
Aug 21, 2021·Scientific Reports·Chaisak ChansriniyomOnanong Nuchuchua
Aug 29, 2021·Pharmaceutics·Xudong TangQingrong Huang
Sep 26, 2021·Clinical and Experimental Immunology·Alessandra BettiolClaudia Fiorillo
Oct 19, 2021·ACS Nano·Ming-Chen SunYong-Zhong Du