ROS-Mediated Cell Cycle Arrest and Apoptosis Induced by Zearalenone in Mouse Sertoli Cells via ER Stress and the ATP/AMPK Pathway

Toxins
Wang-Long ZhengJian-Chun Bian

Abstract

Zearalenone (ZEA) can perturb the differentiation of cells, reduce the generation of reproductive cells and induce a death of germ cells, but the molecular mechanism remains unclear. In order to investigate the potential mechanism of ZEA-induced cell cycle arrest and apoptosis, we studied the effects of ZEA on cell proliferation, cell-cycle distribution, cell-cycle-related proteins, cell death, cell apoptosis, ROS generation and the ATP/AMPK pathway in Sertoli cells. The role of ROS, ER stress and the ATP/AMPK pathway in ZEA-induced cell-cycle arrest and cell apoptosis was explored by using the antioxidant NAC, ER stress inhibitor 4-PBA and the AMPK inhibitor dorsomorphin, respectively. The results revealed that ZEA inhibited the cell proliferation, influenced the distribution of the cell cycle and induced cell apoptosis through the ATP/AMPK pathway. The ATP/AMPK pathway was regulated by ER stress that was induced by ROS generation after exposure to ZEA. Taking these together, this study provided evidence that ROS regulated the process of ZEA-induced cell cycle arrest and cell apoptosis through ER stress and the ATP/AMPK signal ways.

References

May 4, 2005·Molecular Cell·Russell G JonesCraig B Thompson
Apr 15, 2006·The Journal of Physiology·Hiroyuki MotoshimaEiichi Araki
Sep 5, 2009·Toxicology Mechanisms and Methods·Roya KhosrokhavarMir-Jamal Hosseini
Feb 12, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Qing LiuJianchun Bian
Feb 18, 2014·Toxicology Letters·Yajun WangJianchun Bian
Nov 27, 2014·The Journal of Biological Chemistry·Pilar López-CotareloJosé Luis Rodríguez-Fernández
Aug 27, 2015·International Journal of Molecular Sciences·Fenglei ChenYaping Jin
Jan 3, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Fenglei ChenYaping Jin
Feb 7, 2016·Environmental Toxicology and Pharmacology·WangLong ZhengJian Chun Bian
Jul 29, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Elena TatayMaria-Jose Ruiz
May 10, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Elena TatayMaría-José Ruiz
Mar 1, 2015·Comprehensive Reviews in Food Science and Food Safety·Samina Ashiq

❮ Previous
Next ❯

Citations

Aug 27, 2019·Critical Reviews in Food Science and Nutrition·Ankita RaiAnurag Tripathi
Jan 31, 2020·Biofouling·Alwar Ramanujam PadmavathiSubba Rao Toleti
Apr 3, 2020·Toxins·Karolina KowalskaAgnieszka Wanda Piastowska-Ciesielska
May 5, 2018·Toxins·Wanglong ZhengZongping Liu
Feb 6, 2019·International Journal of Molecular Sciences·Mingyang WangPeng Li
Jul 19, 2019·Cell Death & Disease·Fei-Da NiWan-Xi Yang
Aug 7, 2019·The World Journal of Men's Health·Keshab Kumar KarnaJong Kwan Park
Jul 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peirong CaiJianchun Bian
May 27, 2020·Journal of Toxicology·Piyaporn SurinlertChittipong Tipbunjong
Apr 4, 2021·Toxins·Cristina Valeria BulgaruIonelia Taranu
Apr 10, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Baicheng MaLei Yang
Apr 30, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Si ChenPeng Li
May 12, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mingyang WangYi Zhang
May 31, 2021·Ecotoxicology and Environmental Safety·Peirong CaiJianchun Bian
Jun 18, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chengni JinTianming Hu
Mar 7, 2020·Ecotoxicology and Environmental Safety·YanYan YiHongquan Li

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
electron microscopy
protein assay

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved