Roscovitine increases intracellular calcium release and capacitative calcium entry in PC12 cells

Neuroscience Letters
Ho Sook Choi, Sul-Hee Chung

Abstract

Cyclin-dependent kinase 5 (Cdk5), which is activated by the non-cyclin regulator p35 or p39, is a proline-directed serine/threonine kinase that is implicated in many physiological and pathological processes. Here, we studied calcium signaling using the fluorescent cytosolic calcium indicator, Fura-4, in NGF-differentiated PC12 cells treated with roscovitine, a Cdk5 inhibitor. As compared to the control cells, the roscovitine-treated cells significantly potentiated intracellular calcium release by membrane depolarization (high K(+)) or through thapsigargin. In addition, roscovitine increased the magnitude of capacitative calcium entry (CCE), i.e., a calcium influx mechanism triggered by the depletion of intracellular calcium stores. Notably, roscovitine markedly slowed the rate of Ca(2+) removal from the plasma membrane. These results suggest that Cdk5 regulates intracellular calcium homeostasis and that the dysregulation of Cdk5 may contribute to disease pathogenesis by perturbing cellular Ca(2+) signaling.

References

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May 8, 2009·Molecular Neurodegeneration·Philippe MarambaudValérie Vingtdeux

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Citations

Aug 11, 2016·Cellular and Molecular Life Sciences : CMLS·Rafael Andrés Posada-DuqueGloria Patricia Cardona-Gómez
Oct 6, 2020·Journal of Alzheimer's Disease : JAD·Rafael Andrés Posada-Duque, Gloria Patricia Cardona-Gómez

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