Rosiglitazone attenuates NF-κB-mediated Nox4 upregulation in hyperglycemia-activated endothelial cells.

American Journal of Physiology. Cell Physiology
Clintoria R WilliamsC Michael Hart

Abstract

Vascular complications, a major cause of morbidity and mortality in diabetic patients, are related to hyperglycemia-induced oxidative stress. Previously, we reported that rosiglitazone (RSG) attenuated vascular expression and activity of NADPH oxidases in diabetic mice. The mechanisms underlying these effects remain to be elucidated. We hypothesized that RSG acts directly on endothelial cells to modulate vascular responses in diabetes. To test this hypothesis, human aortic endothelial cells (HAECs) were exposed to normal glucose (NG; 5.6 mmol/l) or high glucose (HG; 30 mmol/l) concentrations. Select HAEC monolayers were treated with RSG, caffeic acid phenethyl ester (CAPE), diphenyleneiodonium (DPI), small interfering (si)RNA (to NF-κB/p65 or Nox4), or Tempol. HG increased the expression and activity of the NADPH oxidase catalytic subunit Nox4 but not Nox1 or Nox2. RSG attenuated HG-induced NF-κB/p65 phosphorylation, nuclear translocation, and binding to the Nox4 promoter. Inhibiting NF-κB with CAPE or siNF-κB/p65 also reduced HG-induced Nox4 expression and activity. HG-induced H(2)O(2) production was attenuated by siRNA-mediated knockdown of Nox4, and HG-induced HAEC monocyte adhesion was attenuated by treatment with RSG, DPI,...Continue Reading

References

Aug 1, 1992·The American Journal of Physiology·B Tesfamariam, R A Cohen
Mar 1, 1996·Journal of the American College of Cardiology·S B WilliamsM A Creager
Oct 8, 1997·The American Journal of Physiology·D GiuglianoF D'Onofrio
Jul 10, 1999·Journal of the American College of Cardiology·H KawanoH Yasue
Feb 13, 2001·Endocrine Reviews·J Calles-Escandon, M Cipolla
Jul 18, 2002·Cardiovascular Diabetology·Doron Aronson, Elliot J Rayfield
Apr 21, 2005·Kidney International·Michael S WolinSachin A Gupte
Oct 31, 2006·Oncogene·K De BosscherG Haegeman
Dec 21, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Christian Rask-Madsen, George L King
Jan 24, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Alejandra San MartínKathy K Griendling
Jan 24, 2007·Physiological Reviews·Karen Bedard, Karl-Heinz Krause
Sep 13, 2007·JAMA : the Journal of the American Medical Association·A Michael LincoffSteven E Nissen
Oct 6, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Giulio CeolottoAngelo Avogaro
Nov 6, 2007·The American Journal of Pathology·Young-Mi GoDean P Jones
Dec 26, 2007·Archives of Physiology and Biochemistry·A ManeaM Raicu
Mar 1, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Adrian ManeaMaya Simionescu
Aug 30, 2008·Heart·Sonal Singh, Curt D Furberg
Jan 31, 2009·Cardiovascular Research·Ling Gao, Giovanni E Mann
Jun 13, 2009·American Journal of Respiratory Cell and Molecular Biology·Rachel E NisbetC Michael Hart
Oct 10, 2009·Pharmacological Reports : PR·Sandip Patel, Dev Santani
Nov 13, 2009·American Journal of Physiology. Renal Physiology·Sarah F KnightJohn D Imig
Nov 17, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Bernard Lassègue, Kathy K Griendling
Dec 8, 2009·Diabetes Research and Clinical Practice·Nigel UnwinDavid Whiting
Apr 16, 2010·Canadian Journal of Physiology and Pharmacology·Garry X Shen
May 12, 2010·Biochemical and Biophysical Research Communications·Adrian ManeaMaya Simionescu
Jun 15, 2010·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sumith Retnamma PanickerChandrasekharan Cheranellore Kartha
Jun 29, 2010·Atherosclerosis·Paresh DandonaHusam Ghanim
Jul 14, 2010·Progress in Cardiovascular Diseases·Andre Pascal KengneStephen MacMahon
Jul 14, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xianghuai LuC Michael Hart
Jan 5, 2011·Cell Metabolism·Rebecca G BakerSankar Ghosh

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Citations

Aug 10, 2012·American Journal of Physiology. Cell Physiology·Katherine J MasseyJeffrey L Garvin
Jul 30, 2014·Trends in Endocrinology and Metabolism : TEM·Grant R Drummond, Christopher G Sobey
Jun 19, 2013·Clinical Science·Yves Gorin, Karen Block
Dec 18, 2014·Clinical Science·Sebastiano SciarrettaJunichi Sadoshima
Oct 23, 2012·Cellular Signalling·Balachandar VenkatesanBysani Chandrasekar
Aug 12, 2014·Biochemical and Biophysical Research Communications·Hirohisa OnumaHitoshi Ishida
Dec 18, 2016·Journal of Molecular and Cellular Cardiology·Andrey LozhkinMarschall S Runge
Jan 29, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sherry E AdesinaRoy L Sutliff
Jan 24, 2018·Clinical & Experimental Ophthalmology·Binoy AppukuttanJustine R Smith
Jul 29, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Bing YuWei Kong
Mar 29, 2014·Journal of Neurochemistry·Yali WangJian Cheng
Apr 1, 2015·The Journal of Biological Chemistry·Alvaro Souto Padron de FigueiredoAmina El Jamali
May 23, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Bruna Pinheiro PereiraCarla Speroni Ceron
May 23, 2020·The Journal of Experimental Medicine·Aneesha DasguptaPankaj K Singh
Nov 1, 2018·Physiological Reviews·Jesús TejeroMark T Gladwin
Aug 7, 2019·International Journal of Molecular Sciences·Cesar A MezaRobert C Hickner
Feb 23, 2021·American Journal of Physiology. Renal Physiology·Aswathy M CheriyanClintoria R Williams
Aug 18, 2018·Free Radical Biology & Medicine·Aravind T ReddyRaju C Reddy
May 30, 2021·Kidney International·Guibao KeShuangxin Liu
Jun 27, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wu LiuZeYao Tang

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