ROS‑mediated autophagy through the AMPK signaling pathway protects INS‑1 cells from human islet amyloid polypeptide‑induced cytotoxicity.

Molecular Medicine Reports
Guanghao XiaJinfeng Xiao

Abstract

Oligomerization of human islet amyloid polypeptide (hIAPP) is toxic and contributes to progressive reduction of β cell mass in patients with type 2 diabetes mellitus. Autophagy is a highly conserved homeostatic mechanism in eukaryotes. Previous studies have confirmed that hIAPP can promote autophagy in β cells, but the underlying molecular mechanism and cellular regulatory pathway of hIAPP‑induced autophagy remains not fully elucidated. Accumulation of reactive oxygen species (ROS) causes hIAPP induced‑β cell death. At present, little is known about the association between hIAPP‑induced oxidative stress and autophagy in β cells. Therefore, the present study investigated the underlying molecular mechanism and regulatory pathway of hIAPP‑induced autophagy. Transmission electron microscopy was used to observe the number of autophagosome in cells. Cell viability was determined by an MTT test. A 2',7'‑dichlorofluorescin diacetate assay was used to measure the relative levels of reactive ROS. Western blotting was used to detect expression of adenosine monophosphate‑activated protein kinase (AMPK) and autophagic markers p62 and microtubule associated protein 1 light chain 3. The results demonstrated that hIAPP induces autophagy throug...Continue Reading

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G J CooperK B Reid
Jan 1, 1985·Survey and Synthesis of Pathology Research·G KlöppelP U Heitz
Oct 17, 2001·The Journal of Clinical Investigation·G ZhouD E Moller
Aug 5, 2004·The Journal of Clinical Endocrinology and Metabolism·Rebecca L HullSteven E Kahn
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhaohui FengShengkan Jin
Sep 24, 2005·The FEBS Journal·Barbara KonarkowskaGarth J S Cooper
Nov 22, 2005·Acta Pharmacologica Sinica·Ya-Ping YangZheng-Hong Qin
Mar 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Li YuMichael Lenardo
Jul 29, 2006·Autophagy·David C RubinszteinAviva Tolkovsky
Oct 10, 2006·Diabetes, Obesity & Metabolism·Greg SchimmackNicolas Musi
Feb 11, 2010·Cell·Noboru MizushimaBeth Levine
Aug 24, 2010·Trends in Biochemical Sciences·Ruth Scherz-Shouval, Zvulun Elazar
Jan 25, 2011·Nature Cell Biology·Joungmok KimKun-Liang Guan
Jul 12, 2011·Physiological Reviews·Per WestermarkGunilla T Westermark
Aug 24, 2011·The Journal of Biological Chemistry·Guy LasOrian S Shirihai
Sep 12, 2012·Autophagy·Daniel J KlionskyBrian Zuckerbraun
Jan 26, 2013·Diabetes·Safia CostesPeter C Butler
Jul 23, 2013·Free Radical Biology & Medicine·Xin WenYuquan Wei
Jan 24, 2011·Journal of Diabetes Investigation·Shuhei MoritaTokio Sanke
Jul 19, 2014·The Journal of Clinical Investigation·Nayumi ShigiharaHirotaka Watada
May 16, 2017·The Journal of Clinical Endocrinology and Metabolism·An XinSoroku Yagihashi
Jun 11, 2017·Chemico-biological Interactions·Kishor PantSenthil K Venugopal

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Citations

Nov 12, 2020·Frontiers in Cellular Neuroscience·Carmen Torres-EsquivelLourdes Massieu
Feb 7, 2021·International Journal of Molecular Sciences·Natsuki EguchiHirohito Ichii
Mar 26, 2021·Frontiers in Oncology·Alejandra SuaresOmar Coso

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Methods Mentioned

BETA
fluorescence microscopy
MDA
immunoprecipitation assay
protein assay

Software Mentioned

image J
SPSS

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