RosR (Cg1324), a hydrogen peroxide-sensitive MarR-type transcriptional regulator of Corynebacterium glutamicum.

The Journal of Biological Chemistry
Michael BussmannMichael Bott

Abstract

The cg1324 gene (rosR) of Corynebacterium glutamicum encodes a MarR-type transcriptional regulator. By a comparative transcriptome analysis with DNA microarrays of a ΔrosR mutant and the wild type and subsequent EMSAs with purified RosR protein, direct target genes of RosR were identified. The narKGHJI operon, which encodes a nitrate/nitrite transporter and the dissimilatory nitrate reductase complex, was activated by RosR. All other target genes were repressed by RosR. They encode four putative monooxygenases, two putative FMN reductases, a protein of the glutathione S-transferase family, a putative polyisoprenoid-binding protein, and RosR itself. The DNA binding site of RosR was characterized as an 18-bp inverted repeat with the consensus sequence TTGTTGAYRYRTCAACWA. The in vitro DNA binding activity of RosR was reversibly inhibited by the oxidant H(2)O(2). Mutational analysis of the three cysteine residues present in RosR (Cys-64, Cys-92, and Cys-151) showed that these are responsible for the inhibition of DNA binding by H(2)O(2). A deletion mutant (Δcg1322) lacking the putative polyisoprenoid-binding protein showed an increased sensitivity to H(2)O(2), supporting the role of RosR in the oxidative stress response of C. gluta...Continue Reading

References

May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Mar 1, 1997·Journal of Bacteriology·S Vuilleumier
Mar 6, 2002·Applied Microbiology and Biotechnology·S Vuilleumier, M Pagni
Jul 11, 2002·Journal of Bacteriology·Lauren M StancikJoan L Slonczewski
Nov 7, 2002·Archives of Microbiology·Gerald L Newton, Robert C Fahey
May 14, 2003·Applied Microbiology and Biotechnology·M Ikeda, S Nakagawa
Sep 2, 2003·Journal of Biotechnology·Robert GerstmeirBernhard J Eikmanns
Sep 2, 2003·Journal of Biotechnology·Michael Bott, Axel Niebisch
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jun 1, 1993·Current Biology : CB·D J PappinA J Bleasby
Sep 24, 2004·The Journal of Biological Chemistry·Stelian M PopStephen W Ragsdale
Oct 21, 2004·The Journal of Biological Chemistry·Andreas KrugMichael Bott
Mar 3, 2005·Protein Science : a Publication of the Protein Society·Noriko HandaShigeyuki Yokoyama
Jan 13, 2006·Journal of Biotechnology·Volker F WendischWolfgang Wiechert
Apr 6, 2006·Journal of Bacteriology·Julia Wennerhold, Michael Bott
Aug 23, 2006·Journal of Bacteriology·Sergei Korshunov, James A Imlay
Mar 10, 2007·Applied Microbiology and Biotechnology·Taku NishimuraHideaki Yukawa
Mar 24, 2007·Applied Microbiology and Biotechnology·Seiki TakenoMasato Ikeda
May 15, 2007·Journal of Bacteriology·Sarah Schaaf, Michael Bott
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin-Won LeeJohn D Helmann
Nov 28, 2007·Nucleic Acids Research·Robert D FinnAlex Bateman
Feb 7, 2008·Biochimica Et Biophysica Acta·Chris D den Hengst, Mark J Buttner

❮ Previous
Next ❯

Citations

Mar 12, 2011·Chemical Research in Toxicology·Gerardo Ferrer-SuetaAna Denicola
Aug 24, 2012·PloS One·Nadezda MasloboevaHans-Martin Fischer
Nov 3, 2012·Environmental Microbiology·Ewelina MichtaYvonne Mast
Apr 17, 2014·Proteomics·Andreas KüberlMichael Bott
Apr 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Andreas KüberlMichael Bott
Aug 6, 2016·Nucleic Acids Research·Eugen PfeiferJulia Frunzke
Feb 27, 2015·Biological Chemistry·Melanie Hillion, Haike Antelmann
May 10, 2017·Frontiers in Microbiology·Nadja A HenkePetra Peters-Wendisch
Dec 12, 2020·Microorganisms·Tatjana WalterVolker F Wendisch
Jun 22, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Cedric-Farhad DavoudiMichael Bott

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.