Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR‑γ and UCP2

Molecular Medicine Reports
Ling WangMeiman Hong

Abstract

The present study aimed to investigate whether pretreatment with rosuvastatin (RS) can provide cardioprotection in a myocardial ischemia/reperfusion (MI/R) model. The protective effect of RS on myocardial oxygen‑glucose deprivation/reperfusion (OGD/R) injury was also evaluated by upregulating peroxisome proliferator‑activated receptor‑γ (PPAR‑γ). In the present study, MI/R model was established and activities of superoxide dismutase (SOD), lactate dehydrogenase (LDH), creatine kinase‑muscle/brain (CK‑MB), malondialdehyde (MDA), and troponin I/T were measured. The infarct size was measured using Evans blue staining and cell viability was measured by MTT assay. Reactive oxygen species (ROS) levels were assessed by flow cytometry. Caspase‑9, cytochrome c (cyt c), mitochondrial uncoupling protein 2 (UCP2) and PPAR‑γ expression levels were detected by reverse transcription‑quantitative polymerase chain reaction and western blotting. The results indicated that RS increased SOD activity, and decreased LDH, CK‑MB, MDA and troponin I/T activities. The effect of RS was reversed by atractyloside (ATR). RS inhibited myocardial infarct size, downregulated expression of caspase‑9 and cyt c and upregulated expression of UCP2 and PPAR‑γ by inh...Continue Reading

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Methods Mentioned

BETA
MDA
Assay
ELISA
flow cytometry
PCR
total protein extraction
Protein Assay

Software Mentioned

Cell Quest
Image Lab

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