Rotarod motor performance and advanced spinal cord lesion image analysis refine assessment of neurodegeneration in experimental autoimmune encephalomyelitis

Journal of Neuroscience Methods
Robert van den BergCasper C Hoogenraad

Abstract

Experimental autoimmune encephalomyelitis (EAE) is a commonly used experimental model for multiple sclerosis (MS). Experience with this model mainly comes from the field of immunology, while data on its use in studying the neurodegenerative aspects of MS is scarce. The aim of this study is to improve and refine methods to assess neurodegeneration and function in EAE. Using the rotarod, a tool used in neuroscience to monitor motor performance, we evaluated the correlation between motor performance, disease severity as measured using a clinical scale and area covered by inflammatory lesions. The included parameters are highly correlated in a non-linear manner, with motor performance rapidly decreasing in the intermediate values of the clinical scale. The relation between motor performance and histopathological damage is exclusively determined by lesions in the ventral and lateral columns, based on a new method of analysis of the entire spinal cord. Using a set of definitions for distinct disease milestones, we quantified disease duration as well as severity. The rotarod measures motor performance in a more objective and quantitative manner compared to using a clinical score. The outcome shows a strong correlation to the surface a...Continue Reading

References

Jan 5, 2002·Journal of Neuroimmunology·Machteld M J PolflietTimo K van den Berg
Oct 1, 1953·Journal of Neuropathology and Experimental Neurology·H KLUVER, E BARRERA
Jul 22, 2004·Journal of Neuroimmunology·Bigna S BuddebergMartin E Schwab
Sep 13, 2005·Trends in Immunology·Lawrence Steinman, Scott S Zamvil
Oct 4, 2005·Journal of Neuroimmunology·Kandace K FlemingJanet G Marquis
Nov 22, 2005·Neurobiology of Disease·Joong Hee KimSheng-Kwei Song
Nov 30, 2005·Annals of Neurology·Subramaniam Sriram, Israel Steiner
Jan 1, 1932·Proceedings of the National Academy of Sciences of the United States of America·C P Winsor
May 10, 2007·Nature Protocols·Ingunn M Stromnes, Joan M Goverman
Oct 6, 2007·Nature Reviews. Immunology·Alan G Baxter
Jan 23, 2008·Multiple Sclerosis : Clinical and Laboratory Research·Helen TremlettVirginia Devonshire
Jun 28, 2008·Journal of Neuroimmunology·M V JonesP A Calabresi
Mar 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthew D BuddeSheng-Kwei Song
Aug 12, 2009·Multiple Sclerosis : Clinical and Laboratory Research·F HamiltonJ J Evans
Sep 11, 2009·Annals of Neurology·Manuel A Friese, Lars Fugger
Jun 19, 2010·Progress in Neurobiology·Eilhard MixUwe K Zettl
Jan 22, 2011·Trends in Molecular Medicine·Bert A 't HartRobert Weissert
Mar 5, 2011·Multiple Sclerosis : Clinical and Laboratory Research·David BakerSandra Amor
Jan 26, 2012·BMC Neuroscience·Alessandro MassellaLuciana Giardino
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 28, 2012·Nature Neuroscience·Richard M Ransohoff
Sep 25, 2012·Journal of Neuropathology and Experimental Neurology·Mariella ErredeDaniela Virgintino
Nov 17, 2012·Gait & Posture·Michael J SocieJacob J Sosnoff
Dec 25, 2012·Journal of Neurology·Maria A RoccaMassimo Filippi
Apr 24, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Riley Bove, Tanuja Chitnis
Jun 6, 2013·Mediators of Inflammation·David H TianGila Moalem-Taylor
Jul 23, 2013·PLoS Biology·Konstantinos K TsilidisJohn P A Ioannidis
Jan 7, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Tanya Verma, Julien Cohen-Adad
Apr 1, 2012·International Journal of MS Care·Melanie FlegelDarren Nickel
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sienmi DuRhonda R Voskuhl
Feb 25, 2014·Multiple Sclerosis : Clinical and Laboratory Research·Riley Bove, Tanuja Chitnis
Dec 3, 2014·Proceedings of the National Academy of Sciences of the United States of America·Spencer M MooreSeema Kaushalya Tiwari-Woodruff
Feb 18, 2015·Nature Reviews. Neurology·Richard M RansohoffClaudia F Lucchinetti
Mar 18, 2015·Current Pharmaceutical Design·David Baker, Sandra Amor
Apr 22, 2015·Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging·M Mateo Paz SoldánAaron J Johnson

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