Abstract
We investigated the expression and functionality of a previously developed adenoviral vector carrying the rat cDNA for the dopamine D2 receptor (D2R), AdCMV.DopD2R. Comparative analysis of the autoradiographic images from the striatum injected with AdCMV.DopD2R and the contralateral striatum injected with a control vector, AdCMV.Null, in male rats indicated that D2R binding was increased by 40-60% on days 3 and 5 after injection, but then declined to baseline levels by day 21. When injected with apomorphine on days 3 and 7 after vector injection, experimental groups that had received unilateral striatal injections of AdCMV.DopD2R exhibited a distinct and significant laterality in rotational behavior. These results provide the first demonstration of an adenovirally mediated, intracerebral delivery of a functional neurotransmitter receptor.
References
Jan 1, 1980·Neuroscience·C J Pycock
Oct 1, 1993·Archives of Neurology·P Seeman
Mar 1, 1997·Experimental Neurology·B L Davidson, M C Bohn
Citations
Feb 11, 1999·Journal of Neuroscience Research·G M Smith, M I Romero
Jan 17, 2002·Synapse·Hiroyuki UmegakiMichio Senda
Aug 24, 2004·Cell and Tissue Research·O Eberhardt, J B Schulz
May 9, 2009·Age·Hiroyuki UmegakiDonald K Ingram
May 3, 2005·Life Sciences·Panayotis K ThanosNora D Volkow
Sep 21, 2000·Mechanisms of Ageing and Development·D K IngramH Ikari
Sep 14, 2000·Neuroscience Letters·A H WongH H Van Tol
Apr 20, 2001·Neuroscience·H UmegakiD K Ingram
Oct 3, 2003·Neuroscience·H UmegakiA Iguchi
Feb 10, 1999·Experimental Gerontology·D K IngramG S Roth
Sep 13, 2001·Journal of Neurochemistry·P K ThanosR Hitzemann
Jun 9, 2001·Thyroid : Official Journal of the American Thyroid Association·D H ShenS M Jhian
Mar 12, 2008·Reviews in the Neurosciences·Susanne NikolausHans-Wilhelm Müller
Sep 17, 1998·FEBS Letters·M KnappH H Van Tol
Jun 24, 2008·Neuroscience·T FalkS J Sherman
May 29, 2004·Alcoholism, Clinical and Experimental Research·Panayotis K ThanosNora D Volkow
Apr 18, 2008·Synapse·Panayotis K ThanosNora D Volkow
Aug 31, 2006·Mechanisms of Ageing and Development·Hiroyuki UmegakiAkihisa Iguchi