Rotational mechanism of Enterococcus hirae V1-ATPase by crystal-structure and single-molecule analyses

Current Opinion in Structural Biology
Ryota IinoTakeshi Murata

Abstract

In ion-transporting rotary ATPases, the mechanical rotation of inner rotor subunits against other stator subunits in the complex mediates conversion of chemical free energy from ATP hydrolysis into electrochemical potential by pumping ions across the cell membrane. To fully understand the rotational mechanism of energy conversion, it is essential to analyze a target sample by multiple advanced methods that differ in spatiotemporal resolutions and sample environments. Here, we describe such a strategy applied to the water-soluble V1 moiety of Enterococcus hirae V-ATPase; this strategy involves integration of crystal structure studies and single-molecule analysis of rotary dynamics and torque generation. In addition, we describe our current model of the chemo-mechanical coupling scheme obtained by this approach, as well as future prospects.

References

Jan 1, 1997·Annual Review of Biochemistry·P D Boyer
Oct 17, 1998·The EMBO Journal·G Kaim, P Dimroth
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anja C GemperliJulia Steuber
Apr 3, 2003·The Journal of Biological Chemistry·Tomoyuki HirataMasamitsu Futai
Sep 26, 2003·Biophysical Journal·Markus DittrichKlaus Schulten
Apr 2, 2005·Science·Takeshi MurataJohn E Walker
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hiromi ImamuraKen Yokoyama
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Nobuyasu Koga, Shoji Takada
Nov 4, 2006·The EMBO Journal·Venkataraman KabaleeswaranDavid M Mueller
Jun 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Takeshi MurataSo Iwata
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daichi OkunoHiroyuki Noji
Feb 24, 2009·The Journal of Biological Chemistry·Venkataraman KabaleeswaranDavid M Mueller
Sep 28, 2010·Physical Review Letters·Kumiko HayashiHiroyuki Noji
Sep 28, 2010·Nature Chemical Biology·Rikiya WatanabeHiroyuki Noji
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·David M ReesJohn E Walker
Jan 30, 2013·Biochemical Society Transactions·John E Walker
Feb 12, 2013·Current Opinion in Structural Biology·Ryota Iino, Hiroyuki Noji
Oct 4, 2013·The Journal of Biological Chemistry·Yoshihiro MinagawaRyota Iino
Jun 1, 2014·Current Opinion in Structural Biology·Alastair G StewartDaniela Stock
Jun 16, 2014·Current Opinion in Structural Biology·Geoffrey K Feld, Matthias Frank
Aug 12, 2014·Science·Martin T J Smith, John L Rubinstein
Sep 18, 2014·IUBMB Life·Ryota IinoTakeshi Murata
Sep 27, 2014·The Journal of Biological Chemistry·Hiroshi UenoRyota Iino
Oct 10, 2014·Chemical Communications : Chem Comm·Gen WatanabeKeisuke Suzuki

❮ Previous
Next ❯

Citations

May 4, 2013·Journal of Molecular Biology·Yumemi NagamatsuKunio Miki
Feb 2, 2018·Cellular and Molecular Life Sciences : CMLS·Hiroshi UenoTakeshi Murata
Nov 4, 2016·Nature Communications·Kano SuzukiTakeshi Murata
Feb 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hendrik SielaffMichael Börsch
Jun 11, 2016·Biochemical Society Transactions·Shaun RawsonStephen P Muench
Feb 8, 2019·Science Advances·Shintaro MaruyamaTakeshi Murata
Feb 26, 2019·Frontiers in Physiology·Abhishek SingharoyTakeshi Murata
Dec 6, 2017·Biophysics Reviews·Toru Ekimoto, Mitsunori Ikeguchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.