PMID: 6172277Nov 1, 1981Paper

Rotational molecular dynamics of the membrane-bound acetylcholine receptor revealed by phosphorescence spectroscopy

European Journal of Biochemistry
M BartholdiT M Jovin

Abstract

The rotational mobility of the membrane-bound acetylcholine receptor from Torpedo marmorata has been studied by phosphorescence anisotropy techniques using eosin-5'-isothiocyanate and eosin-5'-iodoacetamide derivatives of the alpha-neurotoxin of Bungarus multicinctus (alpha-bungarotoxin). Normal membranes, those depleted of nonreceptor peripheral peptides by alkaline treatment, as well as membranes subjected to various chemical modifications of the thiol groups, have been characterized. Rotational correlation times (10--26 mus) compatible with the motion of individual 9-S monomeric receptor species of Mr 250 000 were observed upon reduction of the membranes with dithiothreitol or by raising the temperature to 39 degrees C in the case of alkaline-treated membranes. Membranes prepared throughout in N-ethylmaleimide, which yield upon detergent solubilization a predominant 13-S dimeric species and which are not depleted of the nonreceptor v-peptide (Mr 43 000) by alkaline treatment, are relatively more 'rigid' than normal membranes and do not show the anisotropy components characteristic of the monomeric receptor. The influence of the v-peptide on the structural stability of the receptor oligomeric forms and the thiol-dependent int...Continue Reading

References

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Citations

Dec 1, 1984·Molecular Immunology·T M Jovin
Jan 1, 1995·Biophysical Journal·E TerpetschnigJ R Lakowicz
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F J BarrantesT Wallimann
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S DamjanovichT M Jovin
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H O NghiêmJ P Changeux
Jun 1, 1990·The Journal of Cell Biology·M VelezD Axelrod
Nov 1, 1988·Quarterly Reviews of Biophysics·J MatkóS Damjanovich
Apr 3, 1990·Biochemistry·K M Musier-Forsyth, G G Hammes
Aug 17, 1989·Biochimica Et Biophysica Acta·J M Vanderkooi, J W Berger
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·F J Barrantes
Jan 1, 1985·Journal of Receptor Research·J R Zabrecky, M A Raftery

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