Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy

Biochimica Et Biophysica Acta
J BorejdoT P Burghardt

Abstract

In order to measure the cycling of a few ( approximately 6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.

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Citations

Dec 24, 2010·Biophysics Reviews·Thomas P Burghardt, Katalin Ajtai
May 29, 2007·Analytical Biochemistry·P MuthuJ Borejdo
Jul 18, 2006·Biophysical Journal·J BorejdoI Gryczynski
Aug 15, 2006·International Journal of Biological Macromolecules·Avraham Oplatka
Mar 28, 2006·Biophysical Journal·Thomas P BurghardtKatalin Ajtai
Jan 3, 2009·Methods in Cell Biology·Daniel Axelrod
Dec 1, 2012·Biophysics Reviews·J BorejdoK Midde

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