Rotavirus 2/6 viruslike particles administered intranasally with cholera toxin, Escherichia coli heat-labile toxin (LT), and LT-R192G induce protection from rotavirus challenge.

Journal of Virology
C M O'NealM E Conner

Abstract

We have shown that rotavirus 2/6 viruslike particles composed of proteins VP2 and VP6 (2/6-VLPs) administered to mice intranasally with cholera toxin (CT) induced protection from rotavirus challenge, as measured by virus shedding. Since it is unclear if CT will be approved for human use, we evaluated the adjuvanticity of Escherichia coli heat-labile toxin (LT) and LT-R192G. Mice were inoculated intranasally with 10 microg of 2/6-VLPs combined with CT, LT, or LT-R192G. All three adjuvants induced equivalent geometric mean titers of rotavirus-specific serum antibody and intestinal immunoglobulin G (IgG). Mice inoculated with 2/6-VLPs with LT produced significantly higher titers of intestinal IgA than mice given CT as the adjuvant. All mice inoculated with 2/6-VLPs mixed with LT and LT-R192G were totally protected (100%) from rotavirus challenge, while mice inoculated with 2/6-VLPs mixed with CT showed a mean 91% protection from challenge. The availability of a safe, effective mucosal adjuvant such as LT-R192G will increase the practicality of administering recombinant vaccines mucosally.

References

Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·G DouceG Dougan
Oct 29, 1997·Journal of Virology·C M O'NealM E Conner

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Citations

Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I M BelyakovJ A Berzofsky
Oct 13, 2006·Current Opinion in Gastroenterology·M Ciarlet, M K Estes
May 20, 2011·Journal of Virology·Glendie MarcelinMargaret E Conner
Jan 7, 2006·The Pediatric Infectious Disease Journal·Ulrich DesselbergerTimo Vesikari
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·D I Bernstein
Oct 7, 2006·Current Opinion in Gastroenterology·R D Shaw
Jul 26, 2012·Expert Review of Vaccines·Markus A RoseUlrich Baumann
Apr 1, 1999·Biotechnology and Bioengineering·B JiangH P Madore
Mar 1, 2005·Vaccine·L C Freytag, J D Clements
May 21, 2003·International Journal of Medical Microbiology : IJMM·Per Brandtzaeg

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