Rotavirus double-stranded RNA induces apoptosis and diminishes wound repair in rat intestinal epithelial cells

Journal of Gastroenterology and Hepatology
Akiko SatoSumio Watanabe

Abstract

Recent studies have shown that toll-like receptor 3 (TLR3) recognizes double-stranded RNA (dsRNA). Rotaviruses, having a dsRNA genome, infect intestinal epithelial cells (IEC) and cause acute gastroenteritis in young children. The aim of the present study was to clarify the pathophysiological function of rotavirus dsRNA in IEC. Expression of TLR3 mRNA or protein in IEC cell lines (IEC-6, HT-29, Caco-2) was assessed by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis or immunohistochemistry. Induction of cytokines (TNF-alpha, interferon-beta, interleukin-6) mRNA and activation of signal proteins (ERK1/2 MAPK and IkappaB-alpha) in IEC after stimulation with rotavirus dsRNA were assessed by RT-PCR or Western blot analysis. IEC-6 cells were wounded and cell migration into wound areas after stimulation with rotavirus dsRNA (1-25 microg/mL) was assessed. Induction of apoptosis after stimulation with rotavirus dsRNA was also assessed. Expression of TLR3 mRNA and TLR3 protein was detected in IEC. Expression of TLR3 mRNA in IEC-6 tended to be up-regulated by exposure to IFN-gamma. Induction of cytokine mRNA and activation of the signal proteins were detected after stimulation with rotavirus dsRNA. Apoptosi...Continue Reading

References

Sep 1, 1989·Molecular and Cellular Probes·O NakagomiN Ikegami
Nov 1, 1993·Gastroenterology·C E Pope
Mar 1, 1997·Journal of Virology·K V KiblerB L Jacobs
Mar 27, 1997·Biochemical and Biophysical Research Communications·S YokoyamaK Yagi
Oct 23, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M J Clemens, A Elia
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E CarioD K Podolsky
Aug 30, 2000·Nature·A Aderem, R J Ulevitch
Dec 29, 2000·Nature·H HemmiS Akira
Jul 12, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A P Morris, M K Estes
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A T GewirtzJ L Madara
Mar 6, 2002·The Journal of Experimental Medicine·Mathias W HornefAgneta Richter-Dahlfors
Mar 30, 2002·The Journal of Biological Chemistry·Maria T AbreuMoshe Arditi
Jun 11, 2002·Biochemical and Biophysical Research Communications·Misako MatsumotoTsukasa Seya
Sep 18, 2002·Archives of Medical Research·Carlos F AriasSusana López
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masahiro YamamotoShizuo Akira
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gil MelmedMaria T Abreu
Apr 1, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin Götte
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·P Clarke, K L Tyler
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·M Chawla-SarkarE C Borden
Oct 22, 2003·The Journal of Experimental Medicine·Mathias W HornefStaffan Normark
Nov 11, 2003·Clinical and Diagnostic Laboratory Immunology·B JiangR I Glass
Feb 21, 2004·Science·Sandra S DieboldCaetano Reis e Sousa
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhengfan JiangXiaoxia Li
Apr 28, 2004·Virology·Kurt H EdelmannMichael B A Oldstone

❮ Previous
Next ❯

Citations

Jun 22, 2011·Cancer Immunology, Immunotherapy : CII·Edit KovalcsikMark D Bodman-Smith
Jan 30, 2013·PLoS Pathogens·Michelle M ArnoldJohn T Patton
Jun 3, 2011·World Journal of Gastroenterology : WJG·Masahiro Iizuka, Shiho Konno
Aug 6, 2010·Journal of Autoimmunity·Menelaos N ManoussakisChristos I Maratheftis
Mar 30, 2010·Veterinary Microbiology·Channakeshava Sokke UmeshappaAwadh Bihari Pandey
Jun 20, 2014·Immunological Reviews·Hyeseon Cho, Brian L Kelsall
May 16, 2008·Angewandte Chemie·Mohammed Ibrahim ShukoorWolfgang Tremel
Mar 29, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anastasia N VlasovaLinda J Saif
Aug 13, 2011·Journal of Pediatric Gastroenterology and Nutrition·Takeshi SaitoHideo Yoshida
Oct 21, 2021·Journal of Virology·Mahmoud SolimanSang-Ik Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.