Rotavirus enterotoxin NSP4 binds to the extracellular matrix proteins laminin-beta3 and fibronectin

Journal of Virology
J A BoshuizenA W C Einerhand

Abstract

Rotavirus is the most important cause of viral gastroenteritis and dehydrating diarrhea in young children. Rotavirus nonstructural protein 4 (NSP4) is an enterotoxin that was identified as an important agent in symptomatic rotavirus infection. To identify cellular proteins that interact with NSP4, a two-hybrid technique with Saccharomyces cerevisiae was used. NSP4 cDNA, derived from the human rotavirus strain Wa, was cloned into the yeast shuttle vector pGBKT7. An intestinal cDNA library derived from Caco-2 cells cloned into the yeast shuttle vector pGAD10 was screened for proteins that interact with NSP4. Protein interactions were confirmed in vivo by coimmunoprecipitation and immunohistochemical colocalization. After two-hybrid library screening, we repeatedly isolated cDNAs encoding the extracellular matrix (ECM) protein laminin-beta3 (amino acids [aa] 274 to 878) and a cDNA encoding the ECM protein fibronectin (aa 1755 to 1884). Using deletion mutants of NSP4, we mapped the region of interaction with the ECM proteins between aa 87 and 145. Deletion analysis of laminin-beta3 indicated that the region comprising aa 726 to 875 of laminin-beta3 interacts with NSP4. Interaction of NSP4 with either laminin-beta3 or fibronectin wa...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A QuaroniE Ruoslahti
Jun 1, 1992·The Journal of Pediatrics·M A GilgerM K Estes
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C T ChienS Fields
Sep 1, 1990·Baillière's Clinical Gastroenterology·A F SalimM J Farthing
Jun 1, 1991·The Journal of Cell Biology·J E Schwarzbauer
Dec 15, 1990·The Biochemical Journal·K C InghamD H Atha
Mar 10, 1990·European Journal of Biochemistry·I HunterJ Engel
Nov 1, 1989·Journal of Virology·K S AuM K Estes
Dec 1, 1989·Microbiological Reviews·M K Estes, J Cohen
Mar 1, 1988·Journal of Pediatric Gastroenterology and Nutrition·M P OsborneJ Stephen
Dec 1, 1995·Experimental Cell Research·C ffrench-Constant
Dec 1, 1994·Journal of Molecular Evolution·E LittleR F Doolittle
Jan 1, 1994·Methods in Enzymology·P D Yurchenco, J J O'Rear
May 15, 1994·Structure·I D Campbell, C Spitzfaden
Apr 1, 1994·Experimental Cell Research·C M NiessenA Sonnenberg
Feb 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Timpl, J C Brown
Nov 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I LeivoI Virtanen
Dec 1, 1996·Virchows Archiv : an International Journal of Pathology·H KosmehlD Katenkamp
Feb 12, 1998·The Journal of Biological Chemistry·Y S ChengP D Yurchenco
Feb 3, 1999·Annals of the New York Academy of Sciences·P Simon-AssmannA De Arcangelis

❮ Previous
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Citations

Apr 12, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Susanne SchéelePeter Ekblom
Apr 2, 2005·Viral Immunology·Judith M BallRebecca D Parr
Dec 24, 2011·Acta Crystallographica. Section D, Biological Crystallography·Anita R ChackoRandy J Read
Apr 14, 2012·Journal of Amino Acids·Megan E SchroederJudith M Ball
Nov 14, 2013·Virology Journal·Judith M BallRebecca D Parr
Mar 23, 2007·Virology Journal·Mathie Lorrot, Monique Vasseur
Jul 14, 2012·Current Opinion in Virology·Liya HuB V Venkataram Prasad
Jul 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Neung-Seon SeoMary K Estes
Feb 4, 2012·Trends in Microbiology·Lennert SteukersHans J Nauwynck
Aug 7, 2007·Expert Review of Anti-infective Therapy·Robert F Ramig
Jun 26, 2015·Viruses·Ching Wooen Sze, Yee-Joo Tan
Nov 10, 2017·Nature Reviews. Disease Primers·Sue E CrawfordMary K Estes
Feb 20, 2018·Archives of Virology·Sushant KumarK Suguna
Dec 2, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·J Marc RhoadsJack Odle
Feb 18, 2011·Gut Microbes·Kim Hodges, Ravinder Gill
Sep 8, 2015·Current Protocols in Microbiology·Krystle A YaksheJudith M Ball
Apr 21, 2010·Journal of Virological Methods·Carlos A GuerreroOrlando Acosta

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