Rotavirus G and P types circulating in Brazil: characterization by RT-PCR, probe hybridization, and sequence analysis

Archives of Virology
J P LeiteJ R Gentsch

Abstract

We used reverse transcription-polymerase chain reaction (RT-PCR) to determine the P and G genotypes of 130 culture-adapted rotavirus strains isolated from 181 fecal specimens of children under 5 years of age from 9 states and the Federal District of Brazil. The 4 genotypes found most commonly worldwide were also common in Brazil and P[8]G1 was the most prevalent (43%), followed by P[4]G2 (12%), P[8]G3 (6%), and P[8]G4 (6%). However, unusual types P[8]G5, P[6]G2, P[9]G1, P[9]G3, and mixed infections were responsible for 12% and 21% of the cases, respectively. Genotype G5 strains were detected in specimens collected in all 9 areas surveyed from all 4 regions of Brazil. The unusual strain diversity in Brazil suggests that when tetravalent rotavirus vaccines currently being developed are introduced into Brazil, laboratory surveillance will be essential to monitor protection against unusual strains, particularly those of genotype 5, as well as emergence of novel reassortants that may evolve from the large pool of children with mixed infections.

References

Apr 1, 1992·Journal of Clinical Microbiology·P A WoodsR I Glass
Jun 1, 1992·Journal of Clinical Microbiology·J R GentschM K Bhan
Jun 1, 1992·Journal of Clinical Microbiology·G BeardsM A McCrae
Jan 1, 1992·Journal of Clinical Microbiology·G GernaJ Flores
Apr 1, 1992·Immunology and Cell Biology·E J SteeleG W Both
May 1, 1991·Journal of Clinical Microbiology·R F BishopG L Barnes
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M GorzigliaR M Chanock
May 1, 1991·The Journal of General Virology·G F BrowningD R Snodgrass
Jan 1, 1990·Archives of Virology·S UrasawaH Goto
Dec 1, 1989·Journal of Clinical Microbiology·G M BeardsT H Flewett
Jan 1, 1988·Annales De L'Institut Pasteur. Virology·A C LinharesG M Beards
Jan 1, 1985·Journal of Virological Methods·H G PereiraL De Castro
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y HoshinoR M Chanock
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jun 1, 1995·Journal of Clinical Microbiology·A D SteeleM J Mphahlele
Jul 1, 1994·Journal of Clinical Microbiology·B K DasR I Glass
May 1, 1994·Journal of Clinical Microbiology·V GouveaN Santos
Feb 1, 1996·Journal of Clinical Microbiology·M RamachandranJ R Gentsch
Mar 16, 2002·Revista Do Instituto De Medicina Tropical De São Paulo·Alexandre C LinharesJoana D'Arc P Mascarenhas

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Citations

Jul 18, 2000·Journal of Medical Virology·M Iturriza-GómaraJ Gray
Mar 11, 2010·Virus Genes·Kamruddin AhmedOsamu Nakagomi
Jun 1, 2011·Indian Journal of Virology : an Official Organ of Indian Virological Society·Anupam Mukherjee, Mamta Chawla-Sarkar
Aug 10, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Nete Munk NielsenThea Kølsen Fischer
Apr 7, 2004·Veterinary Microbiology·Alice Fernandes AlfieriLeonardo José Richtzenhain
Jul 6, 2013·Veterinary Microbiology·Ha-Hyun KimKyoung-Oh Cho
Sep 25, 2002·Journal of Virological Methods·Kathleen M TattiRoger Glass
Dec 3, 2008·Epidemiology and Infection·J K ZadeS D Chitambar
Apr 2, 2005·Viral Immunology·Judith M BallRebecca D Parr
Dec 12, 2001·The Pediatric Infectious Disease Journal·J S BreseeM Yunus
Oct 27, 2004·The Pediatric Infectious Disease Journal·Alejandro A CastelloJon Gentsch
Jan 7, 2006·The Pediatric Infectious Disease Journal·Ulrich DesselbergerTimo Vesikari
Oct 1, 2010·The Pediatric Infectious Disease Journal·Sylvia Becker-DrepsFélix Espinoza
Nov 5, 2010·The Pediatric Infectious Disease Journal·Filipe Anibal Carvalho-CostaJosé Paulo Gagliardi Leite
Sep 12, 2002·Microbiology and Immunology·Shanti DiwakarlaEnzo A Palombo
May 18, 2007·Journal of Clinical Microbiology·Olga TcheremenskaiaUNKNOWN Rotavirus Study Group
Aug 25, 2006·Journal of Clinical Microbiology·Sung-Hee ParkKyoung-Oh Cho
Sep 28, 2001·Journal of Clinical Microbiology·V JainUNKNOWN Indian Strain Surveillance Collaborating Laboratories
Mar 7, 2001·Journal of Clinical Microbiology·N A CunliffeC A Hart
Apr 28, 2001·Journal of Clinical Microbiology·R H AsmahF Cutts
May 1, 2002·Journal of Clinical Microbiology·Zhao-Yin FangJon R Gentsch
Aug 1, 2002·Journal of Clinical Microbiology·Maria Luzia Rosa E SilvaVera Gouvea
Jul 5, 2003·Journal of Clinical Microbiology·Felipe Mota-HernándezFernando I Puerto
Jul 5, 2003·Journal of Clinical Microbiology·Norma SantosYasutaka Hoshino
Jan 13, 2004·Journal of Clinical Microbiology·Krisztián BányaiGyörgy Szücs
Apr 9, 2004·Journal of Clinical Microbiology·Alicia Sánchez-FauquierEnriqueta Roman
Nov 17, 2012·BMC Infectious Diseases·Andrew McDermidUNKNOWN Members of the Canadian Immunization Monitoring Program, Active
Aug 7, 2012·Virology Journal·Marcelo Takahiro MituiKamruddin Ahmed

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