Rotavirus replication: plus-sense templates for double-stranded RNA synthesis are made in viroplasms

Journal of Virology
Lynn S SilvestriJohn T Patton

Abstract

Rotavirus plus-strand RNAs not only direct protein synthesis but also serve as templates for the synthesis of the segmented double-stranded RNA (dsRNA) genome. In this study, we identified short-interfering RNAs (siRNAs) for viral genes 5, 8, and 9 that suppressed the expression of NSP1, a nonessential protein; NSP2, a component of viral replication factories (viroplasms); and VP7, an outer capsid protein, respectively. The loss of NSP2 expression inhibited viroplasm formation, genome replication, virion assembly, and synthesis of the other viral proteins. In contrast, the loss of VP7 expression had no effect on genome replication; instead, it inhibited only outer-capsid morphogenesis. Similarly, neither genome replication nor any other event of the viral life cycle was affected by the loss of NSP1. The data indicate that plus-strand RNAs templating dsRNA synthesis within viroplasms are not susceptible to siRNA-induced RNase degradation. In contrast, plus-strand RNAs templating protein synthesis in the cytosol are susceptible to degradation and thus are not the likely source of plus-strand RNAs for dsRNA synthesis in viroplasms. Indeed, immunofluorescence analysis of bromouridine (BrU)-labeled RNA made in infected cells provide...Continue Reading

References

May 1, 1990·The Journal of General Virology·J T Patton, C O Gallegos
Nov 1, 1989·Journal of Virology·K S AuM K Estes
Jan 20, 1988·Journal of Molecular Biology·B V PrasadW Chiu
Jan 1, 1984·Virus Research·B L PetrieM K Estes
Jan 1, 1980·The Journal of General Virology·B C AltenburgM K Estes
Apr 1, 1980·The Journal of General Virology·J EsparzaH Römer
Jul 1, 1996·Clinical Microbiology Reviews·K Midthun, A Z Kapikian
Mar 12, 1999·The Journal of General Virology·E FabbrettiO R Burrone
Jan 5, 2000·Genes & Development·T TuschlP A Sharp
Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Mar 15, 2002·Current Opinion in Genetics & Development·György Hutvágner, Phillip D Zamore
Aug 21, 2002·Journal of Virology·Joel W GraffMichele E Hardy
Oct 12, 2002·Archives of Virology·J ChnaidermanE Spencer
Nov 26, 2002·EMBO Reports·Miguel Angel DéctorCarlos F Arias
Jun 6, 2003·The Journal of Biological Chemistry·M Alejandra TortoriciJohn T Patton
Jun 10, 2003·The Journal of Biological Chemistry·Kostas LymperopoulosPolly Roy
Dec 31, 2003·The Journal of Biological Chemistry·Rodrigo Vasquez-Del CarpioZenobia F Taraporewala
Jan 19, 2008·Clinical Microbiology Reviews·Penelope H Dennehy

❮ Previous
Next ❯

Citations

Oct 5, 2006·Archives of Virology·C Piña-VázquezL Padilla-Noriega
Jul 20, 2010·Archives of Virology·Chao FanQin Fang
Jun 21, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Andrea S LaimbacherCornel Fraefel
Jan 24, 2012·Nature Reviews. Microbiology·Shane D TraskJohn T Patton
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shane D TraskJohn T Patton
Apr 2, 2005·Viral Immunology·Judith M BallRebecca D Parr
Feb 12, 2013·Nucleic Acids Research·Leszek LisowskiMark A Kay
Jun 18, 2005·Acta Pharmacologica Sinica·Jun GuoCarlos R Becerra
Jul 29, 2006·Journal of Virology·Zenobia F TaraporewalaJohn T Patton
Aug 31, 2006·Journal of Virology·Takeshi KobayashiTerence S Dermody
Aug 31, 2006·Journal of Virology·Hilda MonteroSusana Lopez
Jul 16, 2010·Journal of Virology·Margarito RojasSusana López
Aug 22, 2008·Journal of Virology·Benno WölkCharles M Rice
Sep 9, 2011·Journal of Virology·Tomás LópezCarlos F Arias
Dec 15, 2004·Journal of Virology·Tomas LópezSusana López
Nov 25, 2005·Journal of Virology·Lynn S SilvestriJohn T Patton
Dec 1, 2010·Virology Journal·José J Carreño-TorresPavel Isa
Jul 22, 2011·Virology Journal·Jose Luis ZambranoMichele E Hardy
Jan 30, 2013·PLoS Pathogens·Michelle M ArnoldJohn T Patton
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Satoshi KomotoKoki Taniguchi
Mar 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mario Barro, John T Patton
Jan 1, 2014·Uirusu·Satoshi Komoto, Koki Taniguchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.