Roughened silver electrodes for use in metal-enhanced fluorescence

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Chris D GeddesJoseph R Lakowicz

Abstract

Roughened silver electrodes are widely used for surface-enhanced Raman scattering (SERS). We tested roughened silver electrodes for metal-enhanced fluorescence. Constant current between two silver electrodes in pure water resulted in the growth of fractal-like structures on the cathode. This electrode was coated with a monolayer of human serum albumin (HSA) protein that had been labeled with a fluorescent dye, indocyanine green (ICG). The fluorescence intensity of ICG-HSA on the roughened electrode increased by approximately 50-fold relative to the unroughened electrode, which was essentially non-fluorescent and increased typically two-fold as compared to the silver anode. No fractal-like structures were observed on the anode. Lifetime measurements showed that at least part of the increased intensity was due to an increased radiative decay rate of ICG. In our opinion, the use of in situ generated roughened silver electrodes will find multifarious applications in analytical chemistry, such as in fluorescence based assays, in an analogous manner to the now widespread use of SERS. To the best of our knowledge this is the first report of roughened silver electrodes for metal-enhanced fluorescence.

Citations

Feb 22, 2008·Analytical Chemistry·Tanya ShtoykoIgnacy Gryczynski
Dec 13, 2006·Journal of Fluorescence·Kadir AslanChris D Geddes
May 19, 2007·Journal of Fluorescence·Yongxia ZhangChris D Geddes
Jan 13, 2009·Chemical Physics Letters·I GryczynskiZ Gryczynski
Jun 22, 2005·Biosensors & Bioelectronics·Victoria V ShumyantsevaClaudio Nicolini
Mar 6, 2012·ACS Nano·Sabrina M WellsMichael J Sepaniak
Apr 22, 2015·Nanoscale Research Letters·Gennadiy GlibitskiyAnatoliy Gasan
May 20, 2009·Analytical and Bioanalytical Chemistry·N CioffiP G Zambonin
May 1, 2015·Scientific Reports·Xiaoying XuKatyayani Seal
Sep 14, 2007·Journal of the American Chemical Society·Ewa M GoldysZygmunt Gryczynski

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