Routine analysis of IgVH mutational status in CLL patients using BIOMED-2 standardized primers and protocols

Leukemia & Lymphoma
Christine MatthewsH Denis Alexander

Abstract

Current methods for the detection of IgVH mutational status in chronic lymphocytic leukemia (CLL), which identifies 2 subgroups of patients with significantly different outcomes, are laborious, expensive and do not lend themselves to a routine diagnostic setting. With the introduction of BIOMED-2 primers, a rapid protocol is now available. This study evaluated the protocol by examining DNA from 100 CLL patients. Conventional methods using RNA, and fluorescence in-situ hybridization (FISH) analysis for recurring chromosomal abnormalities, were carried out on 30 and 60 of these patients, respectively. There was complete concordance between the BIOMED-2 protocol and the RNA based method, both in mutational status and gene usage, whilst unmutated IgVH genes showed association with 17p13 and 11q23 deletions, and trisomy 12, associated with poor and intermediate outcomes, respectively. This study demonstrates that it is feasible to use the BIOMED-2 protocol in the diagnostic profile of CLL patients, obviating the need for inclusion of surrogate markers such as ZAP-70.

Citations

Jan 3, 2006·Pathology Oncology Research : POR·Gábor Méhes
Feb 3, 2009·Haematologica·Eloisa Jantus LewintreJavier García-Conde
Aug 12, 2009·Journal of Hematopathology·Patricia J T A GroenenJohan H J M van Krieken
Nov 24, 2006·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Pietro Maria DonisiVincenzo Stracca-Pansa
Feb 23, 2005·Expert Review of Molecular Diagnostics·Suneel Mundle, Yelina Noskina
Dec 1, 2007·Expert Opinion on Medical Diagnostics·Anton W LangerakJacques Jm van Dongen
Mar 8, 2006·European Journal of Haematology·Mark A CatherwoodH Denis Alexander
Oct 21, 2016·Pathology Oncology Research : POR·Zoltán MátraiTamás Masszi
May 5, 2017·American Journal of Clinical Pathology·Thomas J McDonaldFrank C Kuo
Feb 26, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Krzysztof JamroziakTadeusz Robak
Sep 2, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gema Perez-ChaconJuan M Zapata

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