Routine ribosomal PCR and DNA sequencing for detection and identification of bacteria

Future Microbiology
Michael KempJens Jørgen Christensen

Abstract

Detection and identification of bacteria by PCR and DNA sequencing from clinical sample material has been introduced as a diagnostic routine analysis during the last 5-10 years. Assays analyzing ribosomal genes have been found to be particularly useful. The technique has identified unusual bacteria as well as well-known bacteria in unusual infectious foci. Thereby, it has proven its value both in diagnosing infections in individual patients and as a tool to establish the pathogenic potential of bacteria not previously associated with disease. To be of clinical relevance, results from ribosomal PCR and DNA sequencing must be obtained fast and at acceptable costs. Processing of a high number of samples by individual laboratories can ensure both speed and low price. By continued technical development and further investigations of its usefulness in various clinical settings ribosomal DNA sequencing will most probably become as common a part of clinical bacteriology as culture is today.

References

Jun 1, 1987·Microbiological Reviews·C R Woese
Nov 14, 1997·Journal of Clinical Microbiology·D GoldenbergerM Altwegg
Jan 5, 2000·Journal of Clinical Microbiology·K Rantakokko-JalavaP Kotilainen
Feb 8, 2003·Journal of Clinical Microbiology·Valérie GauduchonFrançois Vandenesch
Mar 5, 2005·The American Journal of Medicine·Gilbert GreubDidier Raoult
Oct 7, 2005·Journal of Clinical Microbiology·Mark ReinhardJens Jørgen Christensen
Oct 13, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jens Jørgen ChristensenMichael Kemp
Jul 25, 2006·Journal of Microbiological Methods·S Mignard, J P Flandrois
Aug 15, 2006·Journal of Microbiological Methods·Oliver GoldenbergUlf B Göbel
Nov 23, 2006·Clinical Oral Investigations·Julio C Machado de OliveiraAlexandre S Rosado
Feb 3, 2007·Journal of Clinical Microbiology·Ulrik S JustesenJens J Christensen
Mar 17, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cathy A Petti
Jul 27, 2007·Scandinavian Journal of Infectious Diseases·Rawaa Jalil Abdul-RedhaMichael Kemp
Aug 19, 2007·Scandinavian Journal of Infectious Diseases·Rawaa Jalil Abdul-RedhaJens Jørgen Christensen
Dec 7, 2007·Journal of Microbiological Methods·Hans-Peter HorzGeorg Conrads
Feb 12, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Michael HandschurAlexander G Haslberger
Apr 2, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Marianne VoldstedlundKurt Fuursted
Apr 19, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cathy A PettiMalliswari Challapalli
Jul 9, 2008·Scandinavian Journal of Infectious Diseases·Kurt FuurstedLisbeth Nørum Pedersen
Sep 16, 2008·The Journal of Emergency Medicine·Wasiulla RafiSursarla Krishna Shankar
Oct 3, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P C Y WooK-Y Yuen
Nov 26, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ann Sofie GleesenMichael Kemp
Dec 17, 2008·Scandinavian Journal of Infectious Diseases·Kristina IblerJens Jørgen Christensen
Dec 18, 2008·The Open Microbiology Journal·Claus MoserJens Jørgen Christensen
Jun 23, 2009·Systematic and Applied Microbiology·Deirdre MikkelsenLindsay I Sly
Jul 9, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Piseth SengDidier Raoult
Dec 26, 2009·Journal of Medical Microbiology·Gitte N Hartmeyer, Ulrik S Justesen
Mar 6, 2010·Emerging Infectious Diseases·Anders KochSteen Villumsen

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Citations

May 11, 2012·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Michael KempJens Jørgen E Christensen
Mar 1, 2012·Forensic Science International. Genetics·Saverio GiampaoliVincenzo Romano Spica
Aug 26, 2015·Injury·Nikolaos K KanakarisPeter V Giannoudis
Nov 20, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sumit PaliwalSamir Mitragotri
Oct 19, 2016·Biosensors & Bioelectronics·Mohsen GolabiAnthony P F Turner

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Methods Mentioned

BETA
PCR
electrophoresis
nucleic acid assays

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