RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp)

Applied and Environmental Microbiology
Jakob BirkeDieter Jendrossek

Abstract

Only two types of rubber oxygenases, rubber oxygenase (RoxA) and latex clearing protein (Lcp), have been described so far. RoxA proteins (RoxAs) are c-type cytochromes of ≈70 kDa produced by Gram-negative rubber-degrading bacteria, and they cleave polyisoprene into 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (ODTD), a C15 oligo-isoprenoid, as the major end product. Lcps are common among Gram-positive rubber degraders and do not share amino acid sequence similarities with RoxAs. Furthermore, Lcps have much smaller molecular masses (≈40 kDa), are b-type cytochromes, and cleave polyisoprene to a mixture of C20, C25, C30, and higher oligo-isoprenoids as end products. In this article, we purified a new type of rubber oxygenase, RoxB Xsp (RoxB of Xanthomonas sp. strain 35Y). RoxB Xsp is distantly related to RoxAs and resembles RoxAs with respect to molecular mass (70.3 kDa for mature protein) and cofactor content (2 c-type hemes). However, RoxB Xsp differs from all currently known RoxAs in having a distinctive product spectrum of C20, C25, C30, and higher oligo-isoprenoids that has been observed only for Lcps so far. Purified RoxB Xsp revealed the highest specific activity of 4.5 U/mg (at 23°C) of all currently known rubber oxy...Continue Reading

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Citations

Jul 12, 2017·Microbial Biotechnology·Wolf RötherDieter Jendrossek
Apr 1, 2018·Biotechnology Progress·R AndlerA Steinbüchel
Nov 1, 2018·Applied Microbiology and Biotechnology·Dieter Jendrossek, Jakob Birke
Jan 11, 2019·Microbiology·Sylvia OetermannAlexander Steinbüchel
May 24, 2019·Applied Microbiology and Biotechnology·Anna CoenenAlexander Steinbüchel
Aug 24, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Stefania Pegorin BragaRenata Castiglioni Pascon
Sep 6, 2019·Applied Microbiology and Biotechnology·Jakob Birke, Dieter Jendrossek
Mar 3, 2020·Bioscience, Biotechnology, and Biochemistry·Daisuke Kasai
Sep 8, 2018·Marine Drugs·Katja GemperleinRolf Müller
Sep 15, 2018·Applied Microbiology and Biotechnology·Jakob BirkeDieter Jendrossek
Jul 19, 2020·Applied Microbiology and Biotechnology·Namiko GibuDaisuke Kasai
Sep 17, 2021·Macromolecular Bioscience·Franciela Arenhart Soares, Alexander Steinbüchel
Oct 8, 2021·The Science of the Total Environment·Hui ZhangJing Wu

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