Royal Jelly Attenuates LPS-Induced Inflammation in BV-2 Microglial Cells through Modulating NF-κ B and p38/JNK Signaling Pathways

Mediators of Inflammation
Meng-Meng YouFu-Liang Hu

Abstract

Royal jelly (RJ), a hive product with versatile pharmacological activities, has been used as a traditional functional food to prevent or treat inflammatory diseases. However, little is known about the anti-inflammatory effect of RJ in microglial cells. The aim of this study is to assess the anti-inflammatory effects of RJ in lipopolysaccharide- (LPS-) induced murine immortalized BV-2 cells and to explore the underlying molecular mechanisms. Our results showed that in LPS-stimulated BV-2 cells, RJ significantly inhibited iNOS and COX-2 expression at mRNA and protein levels. The mRNA expression of IL-6, IL-1β, and TNF-α was also downregulated by RJ in a concentration-dependent manner. Additionally, RJ protected BV-2 cells against oxidative stress by upregulating heme oxygenase-1 (HO-1) expression and by reducing reactive oxygen species (ROS) and nitric oxide (NO) production. Mechanistically, we found that RJ could alleviate inflammatory response in microglia by suppressing the phosphorylation of IκBα, p38, and JNK and by inhibiting the nucleus translocation of NF-κB p65. These findings suggest that RJ might be a promising functional food to delay inflammatory progress by influencing the microglia function.

References

Jan 1, 1982·Acta Neuropathologica·P Eikelenboom, F C Stam
Jan 12, 2000·Oncogene·C S WilliamsR N DuBois
Jun 20, 2000·Neurobiology of Aging·H AkiyamaT Wyss-Coray
Aug 8, 2002·Neuron·Tony Wyss-Coray, Lennart Mucke
Oct 10, 2003·Neuroreport·Rolis Chien-Wei HouKee-Ching G Jeng
Sep 24, 2005·Annals of the New York Academy of Sciences·Rinat TabakmanPhilip Lazarovici
Dec 21, 2006·Nature Reviews. Neuroscience·Michelle L BlockJau-Shyong Hong
Jan 30, 2007·Gastroenterology·Luis A García Rodríguez, Laura Barreales Tolosa
Jul 31, 2007·Neurobiology of Aging·Johanna OjalaTuula Pirttilä
Oct 30, 2008·Journal of Psychopharmacology·D Van DamP P De Deyn
Feb 9, 2010·Journal of Ethnopharmacology·Xin-Yu YangJian-Guang Wang
Mar 9, 2010·CNS & Neurological Disorders Drug Targets·Shweta Mandrekar-Colucci, Gary E Landreth
Aug 14, 2010·Current Drug Targets·Agnieszka Jazwa, Antonio Cuadrado
Feb 3, 2012·Journal of Alzheimer's Disease : JAD·Gui Hua LinJin Tae Hong
Jan 30, 2013·Journal of Pharmacological Sciences·Shinichi WatanabeHiroaki Araki
Dec 25, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Rajapaksha Gedara Prasad Tharanga JayasooriyaGi-Young Kim
Aug 11, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yebo ZhouJin-Song Bian
Nov 20, 2016·Journal of Neuroscience Research·Manuela PennisiVittorio Calabrese
Feb 23, 2017·Annual Review of Immunology·Marco Colonna, Oleg Butovsky
May 30, 2017·Trends in Pharmacological Sciences·Patrik PopracMarian Valko
Jun 12, 2017·Heart, Lung & Circulation·Michael B StokesPeter J Psaltis

❮ Previous
Next ❯

Citations

Nov 11, 2018·Molecular Biology Reports·Rafa S AlmeerAhmed E Abdel Moneim
Jan 16, 2020·International Journal of Molecular Sciences·Saboor AhmadJianke Li
Mar 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yongming PanFuliang Hu
Apr 21, 2020·International Immunopharmacology·Mengmeng YouFuliang Hu
Feb 11, 2021·Nutrients·Nicolas CollazoMiguel A Prieto
Nov 3, 2020·Molecular Biology Reports·Yasin DuranAhsen Yılmaz
Feb 26, 2021·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Ahmad ShabanizadehMohammad Kazemi Arababadi
Jul 30, 2020·European Journal of Pharmacology·Yeon-Joo JuJong Kil Lee
Jul 13, 2021·The Kaohsiung Journal of Medical Sciences·Yu-Hua DingQiang Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
enzyme-linked immunosorbent assay
protein assay
electrophoresis
ELISA

Software Mentioned

GraphPad
GraphPad Prism
Quantity One

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here