RP-HPLC method for the simultaneous quantitation of boeravinone E and boeravinone B in Boerhaavia diffusa extract and its formulation

Natural Product Research
S G BhopeM J Patil

Abstract

A high-performance liquid chromatography method was developed and validated for the simultaneous quantitation of two major rotenoids, boeravinone E and boeravinone B, in Boerhaavia diffusa extract and its formulation. Chromatographic separation was carried out on an Inertsil ODS-3 column by using gradient mobile phase containing 0.1% v/v orthophosphoric acid in water and acetonitrile. The detection was carried out at 276 nm. The method was validated for specificity, precision, accuracy and robustness. The linearity (r(2) = 0.9989 and 0.9991) was found to be in the range of 7.26-35.75 µg mL(-1) and 2.20-11.00 µg mL(-1) for boeravinone E and B, respectively. The percent recovery observed from the extract sample was 95.22-95.83.

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Citations

Feb 5, 2016·Journal of Ethnopharmacology·Kapil S Patil, Sanjivani R Bhalsing
Mar 29, 2014·Phytochemical Analysis : PCA·Khemraj BairwaSanjay Madhukar Jachak
Oct 31, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Gunjan GuhaLazar Mathew
Sep 22, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Gunjan GuhaR Ashok Kumar
Aug 23, 2017·Natural Product Research·Olaoluwa Omosalewa OlaoluwaOludoyin Adeseun Adigun
Aug 14, 2015·Journal of Dietary Supplements· Prathapan Ayyappan Raghu Kozhiparambil Gopalan

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