Abstract
Reversed phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/MS-APCI) was used to analyze both synthetic triacylglycerols (TAG) having 1-3 branched fatty acids (FA) in the molecule, and natural TAG prepared by precursor directed biosynthesis from valine, leucine and isoleucine and the corresponding branched short-chain acids in cultivations of Rhodococcus erythropolis. The technique made it possible to identify and quantify TAG differing in a single branched-chain FA. Altogether 11 TAG were synthesized, out of which 8 were synthesized stereospecifically. Branched- and straight-chain-TAG were separated and identified while TAG differing only in iso or anteiso FA could not be separated. The APCI mass spectra of iso-, anteiso- and straight-chain TAG were completely identical. The natural material was found to contain 19 TAG having at least one branched FA. Cultivation on six different substrates showed, apart from the presumed and common incorporation of precursors to iso-even, iso-odd and anteiso FA, also some unusual features such as an increase in the content of odd-FA after the addition of Val (attributed to catabolism of Val to propionate) or the appearance of branched monounsaturat...Continue Reading
References
May 27, 1988·Journal of Chromatography·P A MetzW J Van-Denheuvel
Nov 13, 1987·Journal of Chromatography·T Rezanka, P Mares
Jun 1, 1996·Archives of Microbiology·H M AlvarezA Steinbüchel
Jun 1, 2000·Microbiology·M WältermannA Steinbüchel
Aug 19, 2000·Journal of Chromatography. a·O SchaafN J Oldham
Sep 15, 2001·Journal of Chromatography. a·H R Mottram, R P Evershed
Jan 30, 2002·Rapid Communications in Mass Spectrometry : RCM·Wm Craig Byrdwell, William E Neff
May 4, 2002·Microbiology·Héctor M AlvarezAlexander Steinbüchel
Dec 6, 2002·Applied Microbiology and Biotechnology·H M Alvarez, A Steinbüchel
Jan 28, 2004·Rapid Communications in Mass Spectrometry : RCM·Laëtitia FauconnotFabiola Dionisi
Apr 14, 2004·Journal of Applied Microbiology·H C BeckF R Lauritsen
Jul 10, 2004·Journal of Nutritional Science and Vitaminology·Sawitree WongtangtintharnTakayoshi Toda
Jan 13, 2005·Journal of the American Chemical Society·Helge B BodeRolf Müller
Jan 26, 2005·FEMS Microbiology Letters·Hans C Beck
May 20, 2005·Journal of Bacteriology·Marc Wältermann, Alexander Steinbüchel
Jul 21, 2005·Organic & Biomolecular Chemistry·Jeroen S DickschatStefan Schulz
Dec 24, 2005·Journal of the American College of Nutrition·Peter W Parodi
Feb 20, 2007·Journal of Chromatography. a·Miroslav LísaNadezda Kabátová
Feb 23, 2007·Journal of Separation Science·Svetlana MomchilovaArnis Kuksis
Jun 26, 2007·Rapid Communications in Mass Spectrometry : RCM·Heidi LeskinenHeikki Kallio
May 1, 2008·Journal of Applied Microbiology·J DherbécourtA Thierry
Jul 25, 2008·Journal of Industrial Microbiology & Biotechnology·Srdjan JoveticGiancarlo Lancini
Dec 17, 2008·BMC Genomics·Martín A HernándezHéctor M Alvarez
Feb 9, 2010·Chemistry and Physics of Lipids·Tomás RezankaKarel Sigler
Citations
May 23, 2012·Folia Microbiologica·Irena KolouchováTomáš Rezanka
Jul 6, 2012·Folia Microbiologica·Tomáš ŘezankaKarel Sigler
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Alexandre A ShvartsburgThomas O Metz
Jan 10, 2013·Analytical Chemistry·Miroslav Lísa, Michal Holčapek
Mar 22, 2014·Genome Announcements·Hynek StrnadCestmir Vlcek
Sep 14, 2011·Phytochemistry·Tomáš RezankaKarel Sigler
Aug 13, 2011·Journal of Chromatography. a·Miroslav LísaMichal Holčapek
May 24, 2011·Phytochemistry·Tomáš RezankaKarel Sigler
May 23, 2013·Letters in Applied Microbiology·Y LuL Cao
Jul 1, 2015·Lipids·Tomáš ŘezankaKarel Sigler
Jun 19, 2016·Analytical Biochemistry·Tomáš ŘezankaKarel Sigler
Aug 22, 2013·Journal of Separation Science·Tomáš RezankaKarel Sigler
Nov 24, 2020·Journal of Chromatography. a·Andrea Palyzová, Tomáš Řezanka
Dec 18, 2020·Electrophoresis·Andrea PalyzováTomáš Řezanka
May 21, 2021·Journal of the American Society for Mass Spectrometry·Christian FreemanKelly M Hines