RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts.

The EMBO Journal
Pierre LucianoVincent Géli

Abstract

In Saccharomyces cerevisiae, the telomerase complex binds to chromosome ends and is activated in late S-phase through a process coupled to the progression of the replication fork. Here, we show that the single-stranded DNA-binding protein RPA (replication protein A) binds to the two daughter telomeres during telomere replication but only its binding to the leading-strand telomere depends on the Mre11/Rad50/Xrs2 (MRX) complex. We further demonstrate that RPA specifically co-precipitates with yKu, Cdc13 and telomerase. The interaction of RPA with telomerase appears to be mediated by both yKu and the telomerase subunit Est1. Moreover, a mutation in Rfa1 that affects both the interaction with yKu and telomerase reduces the dramatic increase in telomere length of a rif1Δ, rif2Δ double mutant. Finally, we show that the RPA/telomerase association and function are conserved in Schizosaccharomyces pombe. Our results indicate that in both yeasts, RPA directly facilitates telomerase activity at chromosome ends.

References

Oct 21, 1994·Science·M S Singer, D E Gottschling
Dec 1, 1994·Molecular and Cellular Biology·M P LongheseG Lucchini
May 3, 1996·Cell·R J WellingerV A Zakian
Sep 1, 1996·Molecular and Cellular Biology·A K Adams, C Holm
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J Lin, V A Zakian
Aug 26, 1998·Molecular and Cellular Biology·B D BournsV A Zakian
Nov 25, 1998·Nucleic Acids Research·I Dionne, R J Wellinger
Oct 3, 1999·Science·S K Evans, V Lundblad
Jul 19, 2000·Current Biology : CB·T R HughesV Lundblad
Oct 25, 2000·Molecular and Cellular Biology·N GrandinM Charbonneau
Jan 4, 2001·Nature Genetics·S E PetersonD E Gottschling
Mar 7, 2001·Genes & Development·A ChandraV Lundblad
Aug 10, 2002·Science·Andrew K P TaggartVirginia A Zakian
Nov 5, 2002·Genes & Development·Anita G SetoThomas R Cech
Sep 17, 2003·Genes & Development·Anne E StellwagenDaniel E Gottschling
Jan 1, 2004·Nature Genetics·Vera SchramkeVincent Géli
Apr 15, 2004·Molecular and Cellular Biology·Svetlana MakovetsElizabeth H Blackburn
Jun 17, 2004·Genes & Development·Michel LarrivéeRaymund J Wellinger
Jul 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·David C Zappulla, Thomas R Cech
Jul 10, 2004·Current Biology : CB·Alain T DandjinouRaymund J Wellinger
Oct 21, 2004·Molecular Cell·Robin M Ricke, Anja-Katrin Bielinsky
Nov 9, 2004·Nature Structural & Molecular Biology·Timothy S FisherVirginia A Zakian
Dec 2, 2004·Molecular and Cellular Biology·Daniel L Levy, Elizabeth H Blackburn
Aug 13, 2005·The EMBO Journal·Kyle M MillerJulia Promisel Cooper
Nov 22, 2005·Nature Structural & Molecular Biology·David C ZappullaThomas R Cech
Jun 13, 2006·Nature Cell Biology·Ruben C PetreacaConstance I Nugent
Oct 5, 2006·Molecular Cell·Momchil D Vodenicharov, Raymund J Wellinger
Oct 31, 2006·Molecular Cell·Christopher J FrankCarol W Greider

❮ Previous
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Citations

Mar 19, 2013·Chromosoma·Cyril Ribeyre, David Shore
Jun 11, 2014·FEBS Letters·Alexander N MalyavkoOlga A Dontsova
Sep 24, 2015·Biomolecules·Sarah A Sabatinos, Susan L Forsburg
Apr 23, 2014·FEMS Microbiology Reviews·Martin Kupiec
Apr 5, 2014·Biochimica Et Biophysica Acta·André MaicherBrian Luke
May 29, 2016·DNA Repair·Carly L Geronimo, Virginia A Zakian
Sep 19, 2014·PLoS Neglected Tropical Diseases·Patrícia Hermes StocoEdmundo Carlos Grisard
Mar 19, 2016·PLoS Genetics·Mariana C GadaletaEishi Noguchi
Jun 9, 2017·International Journal of Molecular Sciences·Sanket Awate, Robert M Brosh
Oct 19, 2017·The Journal of Eukaryotic Microbiology·Raphael Souza PavaniMaria Carolina Elias
Dec 8, 2017·Nature Communications·Ana Teixeira-SilvaSarah A E Lambert
Jun 4, 2019·Frontiers in Oncology·Rafah A A AlnafakhDharani K Hapangama
May 27, 2017·G3 : Genes - Genomes - Genetics·Eva-Maria HolsteinDavid Lydall
Jul 22, 2016·The Journal of Biological Chemistry·Layal SafaCarole Saintomé
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Jee Whu Lee, Eugene Boon Beng Ong
Mar 31, 2020·Biochimica Et Biophysica Acta. General Subjects·Carlos A H FernandesMaria Isabel Nogueira Cano
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Erin BonnellRaymund J Wellinger

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