Rpb3, stoichiometry and sequence determinants of the assembly into yeast RNA polymerase II in vivo.

The Journal of Biological Chemistry
V SvetlovR R Burgess

Abstract

Stoichiometry of the third largest subunit (Rpb3) of the yeast RNA polymerase II is a subject of continuing controversy. In this work we utilized immunoaffinity and nickel-chelate chromatographic techniques to isolate the RNA polymerase II species assembled in vivo in the presence of the His6-tagged and untagged Rpb3. The distribution pattern of tagged and untagged subunits among the RNA polymerase II molecules is consistent with a stoichiometry of 1 Rpb3 polypeptide per molecule of RNA polymerase. Deletion of either alpha-homology region (amino acids 29-55 or 226-267) from the Rpb3 sequence abolished its ability to assemble into RNA polymerase II in vivo.

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Citations

Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S A DarstG Zhang
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·N A Woychik
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·L MinakhinK Severinov
Mar 2, 2006·Molecular Cell·John T ArigoJeffry L Corden
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·B Coulombe, Z F Burton

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