rPbPga1 from Paracoccidioides brasiliensis Activates Mast Cells and Macrophages via NFkB

PLoS Neglected Tropical Diseases
Clarissa X R ValimMaria Célia Jamur

Abstract

The fungus Paracoccidioides brasiliensis is the leading etiological agent of paracoccidioidomycosis (PCM), a systemic granulomatous disease that typically affects the lungs. Cell wall components of P. brasiliensis interact with host cells and influence the pathogenesis of PCM. In yeast, many glycosylphosphatidylinositol (GPI)-anchored proteins are important in the initial contact with the host, mediating host-yeast interactions that culminate with the disease. PbPga1 is a GPI anchored protein located on the surface of the yeast P. brasiliensis that is recognized by sera from PCM patients. Endogenous PbPga1 was localized to the surface of P. brasiliensis yeast cells in the lungs of infected mice using a polyclonal anti-rPbPga1 antibody. Furthermore, macrophages stained with anti-CD38 were associated with P. brasiliensis containing granulomas. Additionally, rPbPga1 activated the transcription factor NFkB in the macrophage cell line Raw 264.7 Luc cells, containing the luciferase gene downstream of the NFkB promoter. After 24 h of incubation with rPbPga1, alveolar macrophages from BALB/c mice were stimulated to release TNF-α, IL-4 and NO. Mast cells, identified by toluidine blue staining, were also associated with P. brasiliensis c...Continue Reading

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Citations

Jul 6, 2016·BMC Immunology·Valéria Cintra Barbosa-LorenziConstance Oliver
Sep 1, 2016·Mediators of Inflammation·Edismauro Garcia Freitas FilhoMaria Célia Jamur
Jun 24, 2017·IEEE Transactions on Nanobioscience·Michael F LohrerGang-Yu Liu
Feb 26, 2019·Experimental Dermatology·Qingqing JiaoMarcus Maurer
Nov 25, 2016·Biological Research·Nguyen Thi XuanNong Van Hai
Jul 3, 2021·Frontiers in Immunology·Mariela JiménezEva Salinas

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Methods Mentioned

BETA
bronchoalveolar lavage
ELISA
scanning electron microscopy
flow cytometry
lavage

Software Mentioned

GraphPad Prism
Cytosoft Blue
GraphPad

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