Rpn4 and proteasome-mediated yeast resistance to ethanol includes regulation of autophagy

Applied Microbiology and Biotechnology
Julia A BubisD S Karpov

Abstract

Distilled spirits production using Saccharomyces cerevisiae requires understanding of the mechanisms of yeast cell response to alcohol stress. Reportedly, specific mutations in genes of the ubiquitin-proteasome system, e.g., RPN4, may result in strains exhibiting hyper-resistance to different alcohols. To study the Rpn4-dependent yeast response to short-term ethanol exposure, we performed a comparative analysis of the wild-type (WT) strain, strain with RPN4 gene deletion (rpn4-Δ), and a mutant strain with decreased proteasome activity and consequent Rpn4 accumulation due to PRE1 deregulation (YPL). The stress resistance tests demonstrated an increased sensitivity of mutant strains to ethanol compared with WT. Comparative proteomics analysis revealed significant differences in molecular responses to ethanol between these strains. GO analysis of proteins upregulated in WT showed enrichments represented by oxidative and heat responses, protein folding/unfolding, and protein degradation. Enrichment of at least one of these responses was not observed in the mutant strains. Moreover, activity of autophagy was not increased in the RPN4 deletion strain upon ethanol stress which agrees with changes in mRNA levels of ATG7 and PRB1 genes ...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S G GrantD Hanahan
May 25, 1990·Nucleic Acids Research·M E SchmittB L Trumpower
May 8, 1999·Molecular Biology of the Cell·I TanidaE Kominami
Jun 22, 2000·Yeast·V V Kushnirov
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Xie, A Varshavsky
Feb 18, 2004·Molecular Cell·Elena Ezhkova, William P Tansey
Feb 21, 2004·Bioinformatics·Robertson Craig, Ronald C Beavis
May 1, 2004·Bioscience, Biotechnology, and Biochemistry·Shunsuke KubotaDai Hirata
Sep 2, 2006·Journal of Proteome Research·Boris ZybailovMichael P Washburn
Jul 9, 2008·Bioinformatics·Darren KessnerParag Mallick
Sep 25, 2008·BMC Genomics·Won-Chul LeeDongsup Kim
Jun 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rammohan NarayanaswamyEdward M Marcotte
Jul 28, 2009·Applied and Environmental Microbiology·Miguel C TeixeiraIsabel Sá-Correia
Aug 6, 2009·Annual Review of Genetics·Congcong He, Daniel J Klionsky
Nov 17, 2009·The International Journal of Biochemistry & Cell Biology·Donghong JuYouming Xie
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Nov 24, 2011·Nucleic Acids Research·J Michael CherryEdith D Wong
Sep 5, 2012·Journal of Cell Science·Therese JacobsonMarkus J Tamás
Oct 11, 2012·PloS One·Denis OstapenkoMark J Solomon
Nov 23, 2012·Nucleic Acids Research·Tianshun GaoYu Xue
Jan 8, 2013·Journal of the American Society for Mass Spectrometry·Anton A GoloborodkoMikhail V Gorshkov
Apr 5, 2013·Biotechnology for Biofuels·Daniel González-RamosJean-Marc G Daran
Feb 28, 2014·Journal of Proteome Research·Mark V IvanovMikhail V Gorshkov
Mar 15, 2014·PloS One·Kuninori SuzukiYoshinori Ohsumi
Sep 23, 2014·Journal of Industrial Microbiology & Biotechnology·A M ZakiD Greetham
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Sep 4, 2015·Cold Spring Harbor Protocols·Jolanda van LeeuwenGuihong Tan
Oct 6, 2015·Nature Biotechnology·James E DahlmanSilvana Konermann
Mar 1, 2016·Frontiers in Microbiology·Elisabet Navarro-TapiaRoberto Pérez-Torrado

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Citations

Nov 5, 2020·International Journal of Molecular Sciences·Daria S SpasskayaDmitry S Karpov
Apr 12, 2021·Biochemistry. Biokhimii︠a︡·Ildar T GabdrakhmanovIrina A Tarasova
Jun 8, 2021·Applied Microbiology and Biotechnology·B A BrandtR Den Haan

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Methods Mentioned

BETA
protein folding
PCR
PCA
ubiquitination
ubuquitination
environmental stress

Software Mentioned

Pathview
Excel
CRISPOR
Cyclone
REVIGO
SciPy Stats
LightCycler® 480
ProteoWizard
MSConvert
STRING

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