rpoB gene sequencing and spoligotyping of multidrug-resistant Mycobacterium tuberculosis isolates from India

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Naga SureshJitendra Nath Pande

Abstract

Multi drug-resistant Mycobacterium tuberculosis (MDR TB) has been well studied in outbreaks in settings of low endemicity in developed countries. However, the characteristics of MDR TB in the community with high endemicity such as India have not been well investigated. Mutations in the 81-bp rifampicin resistance-determining region of the rpoB gene were analyzed by DNA sequencing of 187 M. tuberculosis clinical isolates (149 resistant and 38 sensitive) from different parts of India. 146-Point mutations and two insertions were found in 146 of 149 resistant isolates in seven codons. The most common mutations were in codons 531 (59%), 526 (22%), and 516 (11.5%). Mutations were not found in three (2%) of the resistant isolates. N-terminal sequencing in these isolates showed no mutation at codon V176. None of the drug-susceptible isolates showed any mutation in the 437-bp rpoB gene segment sequenced. Genotypic analysis revealed a total of 80 different spoligotypes. A unique pattern was found in 65 (43.6%) isolates, whereas 84 (56.4%) were in 15 clusters. Comparison with an international spoligotype database showed ST26, Delhi type (18.1%), ST1, Beijing type (9.4%), and ST11 (5.4%), as the most common. The majority of isolates in the...Continue Reading

References

Oct 1, 1995·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·H De BeenhouwerF Portaels
Feb 14, 1996·JAMA : the Journal of the American Medical Association·P J BifaniB N Kreiswirth
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·S SreevatsanJ M Musser
Mar 4, 1998·Diabetes Research and Clinical Practice·P L de Pablos-VelascoJ A Aguilar
Aug 5, 2000·American Journal of Human Genetics·K R EwenS J Foote
Aug 30, 2001·Journal of Clinical Microbiology·A KrüünerG Källenius
Oct 24, 2001·American Journal of Respiratory and Critical Care Medicine·J A CamineroC Martin
Jan 18, 2002·Antimicrobial Agents and Chemotherapy·Noman SiddiqiSeyed E Hasnain
Mar 7, 2002·Journal of Clinical Microbiology·Lishi QianJames T Douglas
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·R BroschS T Cole
May 31, 2002·Journal of Clinical Microbiology·Olga S ToungoussovaDominique A Caugant
Nov 28, 2002·Emerging Infectious Diseases·Ingrid FilliolNalin Rastogi
Jan 9, 2003·Journal of Clinical Microbiology·Ulf R DahleDominique A Caugant
May 8, 2003·Journal of Clinical Microbiology·Jun YueHonghai Wang
Feb 20, 2004·Diagnostic Microbiology and Infectious Disease·N Vijaya BhanuPradeep Seth
Jun 23, 2004·Emerging Infectious Diseases·Urvashi Balbir SinghPradeep Seth
Jan 11, 2005·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christophe SolaNalin Rastogi
Nov 8, 2005·Journal of Clinical Microbiology·Kristin KremerDick van Soolingen

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Citations

Mar 28, 2012·Antimicrobial Agents and Chemotherapy·Ajay PoudelYasuhiko Suzuki
Jun 10, 2010·PloS One·Solomon GhebremichaelGunilla Källenius
Jul 16, 2015·Postgraduate Medicine·Leyla SahebiSiamak Sabour
Jan 26, 2016·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Khin Saw AyeYasuhiko Suzuki
Jun 24, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Pragya SharmaVishwa Mohan Katoch
Feb 24, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Urvashi Balbir SinghJitendra Nath Pande
Oct 31, 2006·Journal of Microbiological Methods·Naga SureshUrvashi Balbir Singh
Feb 12, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Biljo V JosephSathish Mundayoor
Feb 5, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Fabio LucianiMark M Tanaka
Dec 29, 2013·The Journal of Molecular Diagnostics : JMD·Brent C Satterfield
May 25, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C YaoW Fu
Mar 16, 2007·The Journal of Antimicrobial Chemotherapy·Raphael C Y ChanAugustine F B Cheng
Oct 17, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Husain PoonawalaSharon J Peacock
May 18, 2019·Journal of Global Antimicrobial Resistance·Feriel BouzianeUNKNOWN CNR-MyRMA
Jul 11, 2021·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Mehmood QadirRani Faryal

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