RpoN of the fish pathogen Vibrio (Listonella) anguillarum is essential for flagellum production and virulence by the water-borne but not intraperitoneal route of inoculation.

Microbiology
R O'TooleH Wolf-Watz

Abstract

To investigate the involvement of RpoN in flagellum production and pathogenicity of Vibrio (Listonella) anguillarum, the rpoN gene was cloned and sequenced. The deduced product of the rpoN gene displayed strong homology to the alternative sigma 54 factor (RpoN) of numerous species of bacteria. In addition, partial sequencing of rpoN-linked ORFs revealed a marked resemblance to similarly located ORFs in other bacterial species. A polar insertion or an in-frame deletion in the coding region of rpoN abolished expression of the flagellin subunits and resulted in loss of motility. Introduction of the rpoN gene of V. anguillarum or Pseudomonas putida into the rpoN mutants restored flagellation and motility. The rpoN mutants were proficient in the expression of other proposed virulence determinants of V. anguillarum, such as ability to grow under low available iron conditions, and expression of the LPS O-antigen and of haemolytic and proteolytic extracellular products. The infectivity of the rpoN mutants with respect to the wild-type strain was unaffected following intraperitoneal injection of fish but was reduced significantly when fish were immersed in bacteria-containing water. Thus, RpoN does not appear to regulate any factors req...Continue Reading

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Citations

Jun 9, 2000·Molecular Microbiology·B N Lilley, B L Bassler
Jul 12, 2001·Infection and Immunity·F Higa, P H Edelstein
Apr 20, 2006·Infection and Immunity·Jessica L Rock, David R Nelson
Nov 15, 2011·Journal of Bacteriology·Vijayalakshmi S Iyer, Lynn E Hancock
Jun 13, 2000·Journal of Bacteriology·Y K Kim, L L McCarter
Jan 24, 2006·Journal of Bacteriology·Rocío CanalsSusana Merino
Apr 9, 2004·Journal of Clinical Microbiology·Santiago F GonzálezDouglas R Call
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·L L McCarter
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Jorge H Crosa, Christopher T Walsh
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Mark J KazmierczakKathryn J Boor
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Ravi D Barabote, Milton H Saier
Mar 8, 2008·The Journal of General and Applied Microbiology·Fangfang BaiXiao-Hua Zhang
Jun 1, 2011·Fish Pathology·Hiroaki Naka, Jorge H Crosa
Apr 1, 2006·Molecular Microbiology·Susan M ButlerAndrew Camilli
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Nov 15, 2011·Microbial Pathogenesis·Ane Mohn BjellandHenning Sørum
Nov 2, 2004·BMC Microbiology·Steven M DenkinDavid R Nelson
Jan 20, 2007·FEMS Microbiology Letters·Consuelo EsteveJuan M Tomás
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Feb 21, 2006·FEMS Microbiology Letters·Jeanette E BrömsMatthew S Francis
May 4, 2010·PLoS Biology·Laura Gómez-ConsarnauJarone Pinhassi
Jun 14, 2014·PloS One·Hokchai YamNazalan Najimudin

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