RREB1-induced upregulation of the lncRNA AGAP2-AS1 regulates the proliferation and migration of pancreatic cancer partly through suppressing ANKRD1 and ANGPTL4

Cell Death & Disease
Bingqing HuiYan Zhou

Abstract

Long noncoding RNAs (lncRNAs) have been reported to be involved in a variety of human diseases, including cancers. However, their mechanisms have not yet been fully elucidated. We investigated lncRNA changes that may be associated with pancreatic cancer (PC) by analyzing published microarray data, and identified AGAP2-AS1 as a relatively overexpressed lncRNA in PC tissues. qRT-PCR assays were performed to examine expression levels of AGAP2-AS1. MTT assays, colony formation assays, and EdU assays were used to determine the proliferative capacity of cells. Flow cytometry and TUNEL assays were used to study the regulation of AGAP2-AS1 in the cell cycle and apoptosis. Transwell experiments were used to study changes in cell invasion and metastasis, and a nude mouse model was established to assess the effects of AGAP2-AS1 on tumorigenesis in vivo. RNA sequencing was performed to probe AGAP2-AS1-related pathways. Subcellular fractionation and FISH assays were used to determine the distribution of AGAP2-AS1 in PC cells, and RIP and ChIP were used to determine the molecular mechanism of AGAP2-AS1-mediated regulation of potential target genes. Increased expression of AGAP2-AS1 was associated with tumor size and pathological stage progre...Continue Reading

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Citations

Nov 28, 2019·Cell Proliferation·You ShuaiJifeng Feng
May 27, 2021·Transcription·K T Ramya DeviSanjana Prakash Hemdev

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Datasets Mentioned

BETA
GSE16515

Methods Mentioned

BETA
PCR
ChIP
immunoprecipitation
flow cytometry
xenograft
transfection
surgical
biopsy
transfect
electrophoresis

Software Mentioned

RSEM
Pro Plus
Image
Quantity One
SPSS
GraphPad Prism
Prism
ViennaRNA Web Services
NOISeq
HEML

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