PMID: 7537133Nov 1, 1994Paper

rRNA gene restriction patterns as possible taxonomic tools for the genus actinomyces

Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology
O BarsottiJ J Morrier

Abstract

Species delineation in the genus Actinomyces remains unclear, particularly regarding the two taxa, A. naeslundii and A. viscosus. The ribotyping patterns of 64 strains of Actinomyces, representing 8 species and comprising different serotypes, were studied as possible taxonomic tools, using an acetyl-aminofluorene (AAF)-labelled E. coli 16S + 23S rRNA probe. Similarities between patterns were assessed using Jaccard's coefficient and clustering achieved using the unweighted pair-group method with average linkage (UPGMA) on a Macintosh II (Apple, Cupertino, USA) computer. The dendrogram obtained from the ribotypes gave results which were in reasonable agreement with many previous reports: A. bovis, A. gerensceriae, A. israelii, A. meyerii, A. odontolyticus and A. pyogenes were found to be distinct species but the two taxa A. naeslundii and A. viscosus remained unclear. Further investigations, using a larger number of A. naeslundii and A. viscosus strains and other endonucleases, need to be carried out to provide more information concerning the relatedness of these two taxa. Nevertheless, these preliminary results suggested that the Actinomyces chromosome contains multiple rRNA operons which may be used as an epidemiological and ta...Continue Reading

References

Sep 1, 1986·Annales De L'Institut Pasteur. Microbiology·F Grimont, P A Grimont

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Citations

Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M K Yeung

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