Rrp6 Moonlights in an RNA Exosome-Independent Manner to Promote Cell Survival and Gene Expression during Stress.

Cell Reports
Charles WangGuillaume F Chanfreau

Abstract

The nuclear RNA exosome is essential for RNA processing and degradation. Here, we show that the exosome nuclear-specific subunit Rrp6p promotes cell survival during heat stress through the cell wall integrity (CWI) pathway, independently of its catalytic activity or association with the core exosome. Rrp6p exhibits negative genetic interactions with the Slt2/Mpk1p or Paf1p elongation factors required for expression of CWI genes during stress. Overexpression of Rrp6p or of its catalytically inactive or exosome-independent mutants can partially rescue the growth defect of the mpk1Δ mutant and stimulates expression of the Mpk1p target gene FKS2. The rrp6Δ and mpk1Δ mutants show similarities in deficient expression of CWI genes during heat shock, and overexpression of the CWI gene HSP150 can rescue the stress-induced lethality of the mpk1Δrp6Δ mutant. These results demonstrate that Rrp6p moonlights independently from the exosome to ensure proper expression of CWI genes and to promote cell survival during stress.

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P RussoM Makarow
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M C GustinK Davenport
Oct 3, 1999·The EMBO Journal·C AllmangD Tollervey
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Oct 17, 2003·Nature·Sina GhaemmaghamiJonathan S Weissman
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Apr 3, 2007·Nature Protocols·R Daniel Gietz, Robert H Schiestl
Oct 23, 2008·Nucleic Acids Research·Kevin P Callahan, J Scott Butler
Feb 20, 2009·Molecular Biology of the Cell·Amy C GrahamErik D Andrulis
Jun 30, 2011·Advances in Experimental Medicine and Biology·J Scott Butler, Phil Mitchell
Feb 9, 2012·World Journal of Biological Chemistry·Jade Bernstein, Eric A Toth
Jul 13, 2012·Genetics·Roy Parker
Jul 24, 2012·Biochemical Society Transactions·Katherine E SloanNicholas J Watkins
Sep 26, 2013·Genes & Development·Adam VolanakisLidia Vasiljeva
Feb 21, 2014·Molecular Biology of the Cell·Chunyan JinKatrina F Cooper
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Nov 28, 2015·Wiley Interdisciplinary Reviews. RNA·Melanie J Fox, Amber L Mosley
Dec 3, 2016·Nucleic Acids Research·Elizabeth V Wasmuth, Christopher D Lima
Feb 14, 2017·Molecular Cell·Stefan BressonDavid Tollervey
Feb 17, 2017·Genes & Development·John C Zinder, Christopher D Lima
Sep 6, 2017·Trends in Biochemical Sciences·S Branden Van OssKaren M Arndt
Nov 3, 2017·RNA·Derrick J MortonMilo B Fasken
Aug 4, 2019·Nature Biotechnology·Daehwan KimSteven L Salzberg

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Citations

Feb 19, 2021·Biological Reviews of the Cambridge Philosophical Society·Igor StuparevićMichael Primig

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Datasets Mentioned

BETA
GSE140504

Methods Mentioned

BETA
environmental stress
environmental stresses
co-immunoprecipitation
Knockout
PCR
PCRs
in
protein assay
Co-IP

Software Mentioned

count
HISAT2
QuikChange Primer Design
EdgeR
HTseq
GO Term Finder
imageJ
GraphPad

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