RSBP-1 is a membrane-targeting subunit required by the Galpha(q)-specific but not the Galpha(o)-specific R7 regulator of G protein signaling in Caenorhabditis elegans.

Molecular Biology of the Cell
Morwenna Y Porter, Michael R Koelle

Abstract

Regulator of G protein signaling (RGS) proteins inhibit G protein signaling by activating Galpha GTPase activity, but the mechanisms that regulate RGS activity are not well understood. The mammalian R7 binding protein (R7BP) can interact with all members of the R7 family of RGS proteins, and palmitoylation of R7BP can target R7 RGS proteins to the plasma membrane in cultured cells. However, whether endogenous R7 RGS proteins in neurons require R7BP or membrane localization for function remains unclear. We have identified and knocked out the only apparent R7BP homolog in Caenorhabditis elegans, RSBP-1. Genetic studies show that loss of RSBP-1 phenocopies loss of the R7 RGS protein EAT-16, but does not disrupt function of the related R7 RGS protein EGL-10. Biochemical analyses find that EAT-16 coimmunoprecipitates with RSBP-1 and is predominantly plasma membrane-associated, whereas EGL-10 does not coimmunoprecipitate with RSBP-1 and is not predominantly membrane-associated. Mutating the conserved membrane-targeting sequence in RSBP-1 disrupts both the membrane association and function of EAT-16, demonstrating that membrane targeting by RSBP-1 is essential for EAT-16 activity. Our analysis of endogenous R7 RGS proteins in C. elega...Continue Reading

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Citations

Aug 13, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Catherine Hofler, Michael R Koelle
May 30, 2012·Journal of Neurochemistry·Evangelos LiapisVladlen Z Slepak
Dec 1, 2012·International Journal for Parasitology. Drugs and Drug Resistance·Paul McVeighAaron G Maule
Mar 12, 2015·ELife·Patrick LaurentMario de Bono
May 19, 2021·G3 : Genes - Genomes - Genetics·Santosh KumarMichael R Koelle

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Methods Mentioned

BETA
transgenic
protein assay
affinity purification
GTPase
coimmunoprecipitation
immunoprecipitation
pull down
immunoprecipitations

Software Mentioned

BLAST
Lasergene MegAlign
BLASTP
ImageJ

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