RSM based optimization of nutritional conditions for cellulase mediated Saccharification by Bacillus cereus

Journal of Biological Engineering
Fouzia TabssumJaved Iqbal Qazi

Abstract

Cellulases are enzyme which have potential applications in various industries. Researchers are looking for potential cellulolytic bacterial strains for industrial exploitation. In this investigation, cellulase production of Bacillus cereus was explored while attacking poplar twigs. The bacterium was isolated from the gut of freshwater fish, Labeo rohita and identified by 16S rRNA gene sequencing technology. Various nutritional conditions were screened and optimized through response surface methodology. Initially, Plackett-Burman design was used for screening purpose and optimization was conducted through Box-Bhenken design. The maximum cellulase production occurred at 0.5% yeast extract, 0.09% MgSO4, 0.04% peptone, 2% poplar waste biomass, initial medium pH of 9.0, and inoculum size of 2% v/v at 37 °C with agitation speed of 120 rpm for 24 h of submerged fermentation. The proposed model for optimization of cellulase production was found highly significant. The indigenously produced cellulase enzyme was employed for saccharification purpose at 50 °C for various time periods. Maximum total sugars of 31.42 mg/ml were released after 6 h of incubation at 50 °C.The efficiency of this enzyme was compared with commercial cellulase enzy...Continue Reading

References

Jan 1, 1991·Journal of General Microbiology·K Ozaki, S Ito
Nov 18, 1998·Current Opinion in Structural Biology·E A BayerY Shoham
Oct 11, 2003·Biotechnology Advances·M K Bhat
Jan 1, 1997·Biotechnology Advances·M K Bhat, S Bhat
Jun 9, 2005·Current Opinion in Microbiology·Udo Hölker, Jürgen Lenz
Nov 25, 2014·Natural Products and Bioprospecting·Aarti Thakkar, Meenu Saraf

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Citations

Oct 29, 2020·Preparative Biochemistry & Biotechnology·Anam KhalidZaigui Wang
Mar 18, 2021·Biotechnology Reports·Raquel Nascimento da SilvaChristine Lamenha Luna Finkler
Jun 24, 2021·Archives of Microbiology·Lourdes Yaret Ortiz-CortésPeggy Elizabeth Alvarez-Gutiérrez

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Software Mentioned

MEGA
STATISTICA
OFAT
BLAST
CLUSTAL W

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