RT-PCR analysis of Candida albicans ALS gene expression in a hyposalivatory rat model of oral candidiasis and in HIV-positive human patients

Medical Mycology
Clayton B GreenLois L Hoyer

Abstract

ALS gene expression was studied in the hyposalivatory rat model of oral candidiasis and in clinical specimens collected from HIV-positive patients to assess similarities in expression patterns between the model system and clinical isolates. Two Candida albicans strains, SC5314 and OY-2-76, were used in the rat model system and infection progressed for 3 or 5 days. The strains produced similar oral lesions at 3 days. At 5 days, strain OY-2-76 produced more superficial lesions containing relatively more yeast forms compared to invasive hyphal forms observed for strain SC5314. For all infections, the most severe lesions were observed on the tongue and gingiva overlying the mandible. ALS transcripts were easier to detect by RT-PCR later in infection and under other conditions where more fungal cells were present. Expression of ALS1, ALS2, ALS3 and ALS4 was observed in rats infected for 3 days with ALS5 and ALS9 transcripts detected after 5 days of infection. Expression of ALS6 was observed in a single specimen from a 5-day infection while ALS7 transcript was never found. Expression of all ALS genes was observed in oral clinical material collected from HIV-positive patients although ALS6 and ALS7 transcripts required an extra PCR am...Continue Reading

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Citations

Jun 26, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Anna Carolina Borges Pereira CostaAntonio Olavo Cardoso Jorge
Jan 20, 2009·Eukaryotic Cell·Donna M MacCallumFrank C Odds
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·W LaJean Chaffin
Jan 28, 2014·PloS One·Melissa C Garcia-ShermanPeter N Lipke
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May 19, 2011·Molecular Microbiology·Dagmara S DonohueRonnie G Willaert
May 30, 2013·Virulence·Anna C B P CostaAntonio O C Jorge
Aug 20, 2019·Cellular Microbiology·Doureradjou PeroumalNarottam Acharya
Dec 12, 2018·Frontiers in Microbiology·Subramanian MuthamilShunmugiah Karutha Pandian

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