PMID: 9160099May 1, 1997Paper

RT-PCR topography of chronic psoriasis skin based on analysis of T-cell receptor B variable region gene usage

Scandinavian Journal of Immunology
G AhangariH Wigzell

Abstract

Psoriasis is a hyperproliferative inflammatory disease and 70% of patients develop a chronic plaque form. The pathogenesis of psoriasis is not known but evidence exists that T cells play a crucial role. The T cell V-gene receptor repertoire from psoriasis skin (different layers) was compared with peripheral blood T cells by employing RNA polymerase chain reaction (PCR) amplification. T cell receptor (TCR) BV 5.1, 11, 12, 13.1 and 16 were utilized to a significantly higher degree in areas close to the basal layers when compared to CD4+, CD8+ or unfractionated blood T cells from the same patients, whereas only BV11 and 13.1 genes of T cells from deeper layers of the dermis showed such a skewed usage. No biased usage of TCRBV genes was observed in superficial layers or in whole skin. Furthermore, T cell receptor junctional diversity analysed by high resolution gel electrophoresis showed skin psoriatic T cells to be poly- or oligoclonal. In conclusion, we show that TCRBV gene usage from different layers of psoriatic skin has a different pattern compared with the corresponding gene usage in circulating peripheral blood T cells. This pattern may implicate possible skin-associated antigen or superantigens activating a limited number o...Continue Reading

Citations

Sep 21, 2001·Current Rheumatology Reports·P Costello, O FitzGerald
Sep 29, 2009·Trends in Immunology·Helgi ValdimarssonAndrew Johnston
Mar 19, 1999·Immunology Today·J D Bos, M A De Rie

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