PMID: 3215288Dec 1, 1988Paper

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Epidemiology and Infection
H I Thomas, P Morgan-Capner

Abstract

An antiglobulin enzyme-linked immunosorbent assay for rubella-specific IgG1 and IgG3 was adapted to measure antibody avidity by incorporating a mild protein denaturant, diethylamine (DEA), into the serum diluent. Sera were tested at varying dilutions, both with and without DEA, if they contained sufficient specific IgG1 or IgG3. The optical density (OD) was measured and curves were plotted. The highest OD (V) was noted and halved (V/2). The distance between the OD curves at V/2 was measured as the DEA shift value. Sera were examined from people whose sera contained rubella-specific antibodies as a consequence of infection or vaccination in the distant past (24 sera), recent primary rubella (66 sera), symptomatic reinfection (11 sera) or asymptomatic reinfection (64 sera). For specific IgG1 the DEA shift value was less than 0.6 for cases of rubella in the distant past, compared with greater than 0.8 for the first month after primary infection. The maximum DEA shift value for the sera from cases of reinfection was 0.65. No serum from cases of rubella in the distant past contained sufficient specific IgG3 to estimate avidity. The sera collected within 1 month of onset of primary rubella gave DEA shift values greater than 0.7 compa...Continue Reading

References

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Jun 1, 1988·Epidemiology and Infection·H I Thomas, P Morgan-Capner
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Citations

Feb 1, 1991·Journal of Virological Methods·H I Thomas, P Morgan-Capner
Sep 1, 1992·Journal of Virological Methods·H I ThomasJ M Best
Feb 1, 1996·Clinical and Diagnostic Virology·P Hudson, P Morgan-Capner
Aug 1, 1997·Clinical and Diagnostic Virology·B Böttiger, I P Jensen
Dec 24, 1997·Clinical and Diagnostic Virology·M NaritaH Kikuta
Aug 12, 1999·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·E Vardas, S Kreis
Dec 10, 1999·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·H I ThomasP Morgan-Capner
Jun 1, 1990·Epidemiology and Infection·H I Thomas, P Morgan-Capner
Aug 1, 1991·Epidemiology and Infection·J E Cradock-Watson
Apr 1, 1994·Epidemiology and Infection·R E HollimanJ D Johnson
Nov 10, 2006·Journal of Clinical Microbiology·Samira MubarekaGraham A Tipples
Sep 23, 1989·BMJ : British Medical Journal·J M BestE Miller
Jun 22, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·J NedeljkovicC Oker-Blom
Oct 12, 2000·The Journal of Infection·Y AboudyE Mendelson
Mar 17, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Lea HedmanKlaus Hedman
Dec 23, 2006·Journal of Neuroimmunology·Christoph SteiningerTheresia Popow-Kraupp
Jul 1, 1991·The Journal of Infection·G KudesiaH I Thomas
Dec 24, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V HerneP Reedik
Nov 27, 2015·Clinical Microbiology Reviews·Wayne DimechChristelle Vauloup-Fellous
Oct 29, 1993·Annals of the New York Academy of Sciences·P A Brunell
Nov 11, 1989·BMJ : British Medical Journal
Nov 11, 1989·BMJ : British Medical Journal·J Gilbert, G Kudesia
Mar 9, 2006·Clinical and Vaccine Immunology : CVI·Kim M WilsonElizabeth M Dax
Aug 5, 1989·BMJ : British Medical Journal·P Morgan-Capner
Oct 5, 2011·The Journal of Infectious Diseases·Graham A Tipples

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