Ru(bpy)3(2+)/nanoporous silver-based electrochemiluminescence immunosensor for alpha fetoprotein enhanced by gold nanoparticles decorated black carbon intercalated reduced graphene oxide

Scientific Reports
Wenjuan ZhuQin Wei

Abstract

A highly sensitive sandwich-type electrochemiluminescence (ECL) immunosensor was proposed for the quantitative determination of alpha fetoprotein (AFP) using gold nanoparticles decorated black carbon intercalated reduced graphene oxide (Au-rGO@CB) as sensing platform and nanoporous silver (NPS) loaded Ru(bpy)3(2+) as labels. In this work, intercalation of CB inhibited the accumulation of rGO and Au-rGO@CB was firstly used to immobilize primary antibody (Ab1) in ECL system. NPS prepared by the dealloying of binary alloy has high pore volume and surface areas, which was used to load amount of secondary antibodies (Ab2) and Ru(bpy)3(2+), which could greatly enhance the ECL intensity. Under optimal conditions, the designed immunosensor exhibited wider linear range from 0.0001 to 30 ng/mL with a relative lower detection limit of 33 fg/mL for AFP detection. Overall, the designed immunosensor exhibited high sensitivity and selectivity, good repeatability and stability. This proposed method provided a potential application for clinical monitoring of AFP.

References

Nov 25, 2003·Inorganic Chemistry·Mara StaffilaniLuisa De Cola
Jun 19, 2004·Journal of Cancer Research and Clinical Oncology·Lun-Xiu Qin, Zhao-You Tang
Oct 23, 2004·Science·K S NovoselovA A Firsov
Mar 12, 2005·CA: a Cancer Journal for Clinicians·D Max ParkinPaola Pisani
Feb 13, 2007·Cancer Detection and Prevention·Lorinda M WrightClaus J Fimmel
Mar 16, 2007·Journal of Biotechnology·You-Sub Won, Seong-Wook Lee
Aug 16, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·K E SapsfordM G Ancona
Mar 31, 2009·Biosensors & Bioelectronics·Jian-Ding QiuXing-Hua Xia
Aug 25, 2010·ACS Nano·Daniela C MarcanoJames M Tour
May 13, 2011·Journal of the American Chemical Society·Matthew P KleinIlke Arslan
Apr 26, 2014·Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science·John G BrunoTaylor Phillips
Apr 19, 2015·Biosensors & Bioelectronics·Junfeng LiuZhuobin Yuan

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
biosensor
ELISA

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