PMID: 8955396Dec 1, 1996Paper

Rubrerythrin from Clostridium perfringens: cloning of the gene, purification of the protein, and characterization of its superoxide dismutase function

Journal of Bacteriology
Y LehmannM Teuber

Abstract

The food-borne pathogen Clostridium perfringens, which is an obligate anaerobe, showed growth under conditions of oxidative stress. In protein extracts we looked for superoxide dismutase (SOD) activities which might scavenge highly toxic superoxide radicals evolving under such stress conditions. Using the classical assay to detect SOD activity on gels after electrophoresis of C. perfringens proteins, we obtained a pattern of three major bands indicating SOD activity. The protein representing the brightest band was purified by three chromatographic steps. On the basis of 20 amino acids determined from the N terminus of the protein, we designed a degenerate oligonucleotide probe to isolate the corresponding gene. We finally sequenced an open reading frame of 195 amino acids (molecular mass, 21,159 Da) with a strong homology to the Desulfovibrio vulgaris rubrerythrin; therefore, we assumed to have cloned a rubrerythrin gene from C. perfringens, and we named it rbr. The C-terminal region of the newly detected rubrerythrin from C. perfringens contains a characteristic non-heme, non-sulfur iron-binding site -Cys-X-X-Cys-(X)12-Cys-X-X-Cys- similar to that found in rubrerythrin from D. vulgaris. In addition, three -Glu-X-X-His- sequenc...Continue Reading

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Citations

Apr 26, 2002·Molecular Microbiology·Maryta SztukowskaDonald M Kurtz
Jun 10, 2004·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Zhenmei LüYing Xia
Sep 25, 2008·Journal of the National Cancer Institute·Zhiyu LiSavio L C Woo
Jun 9, 2004·Microbiology·Delphine JeanGilles Reysset
Apr 9, 2020·Archives of Microbiology·Viktor P L Eckel, Rudi F Vogel
Jun 23, 2000·Annals of the New York Academy of Sciences·J M Gutteridge, B Halliwell
Apr 24, 2008·Molecular Microbiology·Falk HillmannHubert Bahl
Jun 12, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John A RavenPatricia Sánchez-Baracaldo
Oct 17, 2001·Journal of Food Protection·J S NovakV K Juneja
Sep 27, 2006·Protein Science : a Publication of the Protein Society·Aditya HindupurRobert O Fox
Apr 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rekha SeshadriIan T Paulsen
Dec 8, 2005·Applied and Environmental Microbiology·Shinji KawasakiYouichi Niimura
Jan 2, 2009·Microbiology·Oliver RiebeHubert Bahl
Mar 2, 1999·Biochemical and Biophysical Research Communications·E D CoulterD M Kurtz
Aug 12, 1999·Fungal Genetics and Biology : FG & B·A LeonowiczJ Rogalski
Jul 1, 1997·Journal of Bacteriology·H L LumppioD M Kurtz
Jun 5, 2007·Journal of Bacteriology·Aindrila MukhopadhyayJay D Keasling
Mar 1, 2006·Journal of Inorganic Biochemistry·Donald M Kurtz
Sep 4, 2013·Journal of Proteome Research·Wishwas AbhyankarChris G de Koster

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