Rules for biocatalyst and reaction engineering to implement effective, NAD(P)H-dependent, whole cell bioreductions

Biotechnology Advances
Regina KratzerBernd Nidetzky

Abstract

Access to chiral alcohols of high optical purity is today frequently provided by the enzymatic reduction of precursor ketones. However, bioreductions are complicated by the need for reducing equivalents in the form of NAD(P)H. The high price and molecular weight of NAD(P)H necessitate in situ recycling of catalytic quantities, which is mostly accomplished by enzymatic oxidation of a cheap co-substrate. The coupled oxidoreduction can be either performed by free enzymes in solution or by whole cells. Reductase selection, the decision between cell-free and whole cell reduction system, coenzyme recycling mode and reaction conditions represent design options that strongly affect bioreduction efficiency. In this paper, each option was critically scrutinized and decision rules formulated based on well-described literature examples. The development chain was visualized as a decision-tree that can be used to identify the most promising route towards the production of a specific chiral alcohol. General methods, applications and bottlenecks in the set-up are presented and key experiments required to "test" for decision-making attributes are defined. The reduction of o-chloroacetophenone to (S)-1-(2-chlorophenyl)ethanol was used as one exa...Continue Reading

References

Jul 28, 1999·Applied Microbiology and Biotechnology·Y YasoharaS Shimizu
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·R DaugelaviciusD H Bamford
Oct 5, 2002·Biotechnology Progress·Steven D DoigJohn M Woodley
Dec 17, 2002·Current Opinion in Biotechnology·Adrie J J StraathofAndreas Schmid
Jul 3, 2003·Trends in Biotechnology·Veronique AlphandJohn M Woodley
Jul 3, 2003·Applied Microbiology and Biotechnology·M KataokaS Shimizu
Sep 26, 2003·Bioprocess and Biosystems Engineering·S G CullP Angeli
Nov 6, 2004·Nature Biotechnology·François Baneyx, Mirna Mujacic
Dec 21, 2004·Journal of Biotechnology·Hans Peter Sørensen, Kim Kusk Mortensen
Jan 26, 2005·Biochemical Society Transactions·R G Sawers
May 19, 2005·Biotechnology Advances·Joseph Shiloach, Rephael Fass
Dec 22, 2005·Nature Methods·Niraj H Tolia, Leemor Joshua-Tor
Mar 21, 2006·Biomolecular Engineering·Vladimir I Tishkov, Vladimir O Popov
Apr 12, 2006·Applied Microbiology and Biotechnology·Evelyn M Buque-TaboadaLuuk A M van der Wielen
Dec 23, 2006·Trends in Biotechnology·David J Pollard, John M Woodley
Jan 16, 2007·Applied Microbiology and Biotechnology·Rachel Ruizhen Chen
May 9, 2007·Applied Microbiology and Biotechnology·Katja GoldbergAndreas Liese
Sep 11, 2007·Journal of Biotechnology·Kirsten SchroerStephan Lütz
Dec 7, 2007·Accounts of Chemical Research·Jeffrey C MoorePaul N Devine
Jul 11, 2009·Bioorganic & Medicinal Chemistry Letters·Yoshiyuki SatoYoshikazu Iwasawa
Apr 2, 2011·Biotechnology and Bioengineering·Pär TufvessonJohn M Woodley
Apr 16, 2011·Science·Wei LüOliver Einsle
Mar 10, 2015·Trends in Biotechnology·Douglas B KellStephen G Oliver

❮ Previous
Next ❯

Citations

Jun 6, 2019·Journal of Industrial Microbiology & Biotechnology·John M BillingsleyYi Tang
Oct 13, 2019·Chembiochem : a European Journal of Chemical Biology·Lei HuangSelin Kara
Jun 15, 2017·Microbial Cell Factories·Baixue Lin, Yong Tao
Jul 19, 2017·Biotechnology Journal·Marvin KadischAndreas Schmid
Nov 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiangxian YingMeirong Ying
Jul 14, 2019·Green Chemistry : an International Journal and Green Chemistry Resource : GC·Joseline A HouwmanFrancesco G Mutti
Apr 15, 2021·Applied Microbiology and Biotechnology·Feng XueQing Xu
Oct 13, 2021·BMC Biotechnology·Christian RappRegina Kratzer
Dec 10, 2021·Applied Microbiology and Biotechnology·In Jung KimUwe T Bornscheuer

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

BLAST
SciFinder

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

© 2021 Meta ULC. All rights reserved