Rumen and Fecal Microbial Community Structure of Holstein and Jersey Dairy Cows as Affected by Breed, Diet, and Residual Feed Intake

Animals : an Open Access Journal From MDPI
S J NoelO Højberg

Abstract

Identifying factors that influence the composition of the microbial population in the digestive system of dairy cattle will be key in regulating these populations to reduce greenhouse gas emissions. In this study, we analyzed rumen and fecal samples from five high residual feed intake (RFI) Holstein cows, five low RFI Holstein cows, five high RFI Jersey cows and five low RFI Jersey cows, fed either a high-concentrate diet (expected to reduce methane emission) or a high-forage diet. Bacterial communities from both the rumen and feces were profiled using Illumina sequencing on the 16S rRNA gene. Rumen archaeal communities were profiled using Terminal-Restriction Fragment Length Polymorphism (T-RFLP) targeting the mcrA gene. The rumen methanogen community was influenced by breed but not by diet or RFI. The rumen bacterial community was influenced by breed and diet but not by RFI. The fecal bacterial community was influenced by individual animal variation and, to a lesser extent, by breed and diet but not by RFI. Only the bacterial community correlated with methane production. Community differences seen in the rumen were reduced or absent in feces, except in the case of animal-to-animal variation, where differences were more pronou...Continue Reading

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Citations

Sep 27, 2020·Animals : an Open Access Journal From MDPI·Viktoria NeubauerQendrim Zebeli
Mar 4, 2020·Journal of Animal Science and Biotechnology·Bruno G N AndradeLuciana C A Regitano
Aug 8, 2020·Frontiers in Microbiology·Jessica C A FriedersdorffChristopher J Creevey
Feb 2, 2021·Frontiers in Veterinary Science·Sinalo ManiMatthew A Adeleke
Aug 8, 2020·Methods : a Companion to Methods in Enzymology·Thea Os AndersenPhillip B Pope
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Mohammad RaminSophie J Krizsan

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Datasets Mentioned

BETA
PRJNA525989

Methods Mentioned

BETA
PCR

Software Mentioned

UCHIME
FastTree2
QIIME
UPARSE
PERMANOVA
FLASH
ClustalO
R
STAMP
LotuS

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