Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

AMB Express
Lovelia L MamuadSang-Suk Lee

Abstract

Supplementation of appropriate probiotics can improve the health and productivity of ruminants while mitigating environmental methane production. Hence, this study was conducted to determine the effects of Enterococcus faecium SROD on in vitro rumen fermentation, methane concentration, and microbial population structure. Ruminal samples were collected from ruminally cannulated Holstein-Friesian cattle, and 40:60 rice straw to concentrate ratio was used as substrate. Fresh culture of E. faecium SROD at different inclusion rates (0, 0.1%, 0.5%, and 1.0%) were investigated using in vitro rumen fermentation system. Addition of E. faecium SROD had a significant effect on total gas production with the greatest effect observed with 0.1% supplementation; however, there was no significant influence on pH. Supplementation of 0.1% E. faecium SROD resulted in the highest propionate (P = 0.005) but the lowest methane concentration (P = 0.001). In addition, acetate, butyrate, and total VFA concentrations in treatments were comparatively higher than control. Bioinformatics analysis revealed the predominance of the bacterial phyla Bacteroidetes and Firmicutes and the archaeal phylum Euryarchaeota. At the genus level, Prevotella (15-17%) and Me...Continue Reading

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Citations

Dec 10, 2021·Microbiology Resource Announcements·A A KorzhenkovS V Toshchakov

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Datasets Mentioned

BETA
PRJNA505970

Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay

Software Mentioned

RDP
Statistical Analysis Systems ( SAS )

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