Abstract
Background:Rumex dentatus, commonly known as tooth docked, is widely used in traditional system of medicines. Although it is well reported for its biological activities and medicinal value, only few studies have been carried out to assess its anticancer potential. Purpose: This study seeks to evaluate the anticancer activity of leaf extracts of R. dentatus against breast cancer MDA-MB-231 cell line, a triple negative human breast cancer cell line with invasive properties and to identify the molecular targets underlying its mechanism of action. Methods: Cytotoxicity of plant extracts was determined against breast cancer cells, using the MTT assay. Flow cytometry was performed to analyze the changes in cell cycle and apoptotic effect, if any. Cells were also studied for their wound healing and invasive potential as well as for Western blotting of apoptotic genes and nuclear factor-kappaB (NF-κB) pathway. Results: The results revealed that R. dentatus methanol (RM) and chloroform (RC) extracts of R. dentatus had the highest inhibition of cell proliferation in a concentration- and time-dependent manner. This inhibitory effect was found to be linked to arrest of cell cycle at the G0/G1 phase, along with induction of apoptosis and ac...Continue Reading
References
Aug 1, 1973·Cancer·E A LefrakJ A Gottlieb
Jan 31, 1998·The Journal of Clinical Investigation·R C BargouB Dörken
Apr 16, 1999·The Journal of Nutrition·H Mo, C E Elson
Mar 14, 2000·Oncogene·P C CogswellA S Baldwin
Apr 25, 2000·The Journal of Biological Chemistry·R W ClarksonC J Watson
Aug 22, 2001·Journal of Agricultural and Food Chemistry·A YildirimA A Kara
Dec 26, 2001·Oncogene·H Yamaguchi, H G Wang
Apr 12, 2003·The Journal of Biological Chemistry·Gregory HelbigHarikrishna Nakshatri
Dec 17, 2003·Oncogene·Sanjay GuptaHasan Mukhtar
Jul 26, 2005·Annals of Hematology·Katrien VermeulenZwi N Berneman
Dec 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thomas A BuchholzAysegul A Sahin
Jun 21, 2006·The Journal of Biological Chemistry·Gautam SethiBharat B Aggarwal
Aug 22, 2006·Cancer Biology & Therapy·Kageaki KuribayashiWafik S El-Deiry
Apr 29, 2008·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Zhengyu LiYuquan Wei
Mar 21, 2009·Cellular Signalling·Fazlul H SarkarDejuan Kong
May 19, 2009·Trends in Pharmacological Sciences·Gautam Sethi, Vinay Tergaonkar
Oct 20, 2009·Nutrition and Cancer·Cristina P R XavierCristina Pereira-Wilson
Jan 19, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hui ZhangYanxia Han
Apr 7, 2012·Journal of Agricultural and Food Chemistry·Hyun Suk KoEun-Ok Lee
Jul 4, 2012·Cancer Metastasis Reviews·Darshni ViraEri S Srivatsan
Aug 21, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Shu-Yi YinNing-Sun Yang
Dec 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Nida Nayyar FarshoriMaqsood Ahmed Siddiqui
Nov 5, 2014·Advanced Pharmaceutical Bulletin·Elham SafarzadehBehzad Baradaran
Apr 1, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Samira GoldarBehzad Baradaran
Citations
Aug 19, 2020·Journal of Ethnopharmacology·Zimei YangXiufei Gao
Feb 23, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Rasha H ElsayedSayed A Ahmed
Aug 17, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Saima AliRaees Khan