Rumex dentatus Inhibits Cell Proliferation, Arrests Cell Cycle, and Induces Apoptosis in MDA-MB-231 Cells through Suppression of the NF-κB Pathway

Frontiers in Pharmacology
Riffat BatoolTariq Mahmood

Abstract

Background:Rumex dentatus, commonly known as tooth docked, is widely used in traditional system of medicines. Although it is well reported for its biological activities and medicinal value, only few studies have been carried out to assess its anticancer potential. Purpose: This study seeks to evaluate the anticancer activity of leaf extracts of R. dentatus against breast cancer MDA-MB-231 cell line, a triple negative human breast cancer cell line with invasive properties and to identify the molecular targets underlying its mechanism of action. Methods: Cytotoxicity of plant extracts was determined against breast cancer cells, using the MTT assay. Flow cytometry was performed to analyze the changes in cell cycle and apoptotic effect, if any. Cells were also studied for their wound healing and invasive potential as well as for Western blotting of apoptotic genes and nuclear factor-kappaB (NF-κB) pathway. Results: The results revealed that R. dentatus methanol (RM) and chloroform (RC) extracts of R. dentatus had the highest inhibition of cell proliferation in a concentration- and time-dependent manner. This inhibitory effect was found to be linked to arrest of cell cycle at the G0/G1 phase, along with induction of apoptosis and ac...Continue Reading

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Citations

Feb 23, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Rasha H ElsayedSayed A Ahmed
Aug 17, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Saima AliRaees Khan

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Methods Mentioned

BETA
protein assay
ELISA
flow cytometry
ubiquitination

Software Mentioned

ImageJ
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